biostudies-literatureUnknown15(1)Ibrahim RManipal Academy of Higher Education, ManipalPopulation explosion in recent years has driven the environment to overuse nondegradable substances. Microbial polyesters known as polyhydroxyalkanoates (PHAs) are generated and retained as cytoplasmic granules in microorganisms with restricted nutritional availability and can be used to manufacture bioplastics. The current study attempts to screen soil isolates for PHA production and optimize their media parameters. Among all the isolates, 17 were identified and confirmed by Sudan black staining, as they are screening for PHA production and are identified by their colony characteristics. The isolation of the most promising strain, GS-14, was achieved through the sodium hypochlorite method, and subsequent quantification involved establishing a standard curve of crotonic acid. Notably, isolScientific reports8395https://www.ebi.ac.uk/biostudies/studies/S-EPMC11897369biostudies-literatureProduction, isolation, optimization, and characterization of microbial PHA from Bacillus australimaris.PMC11897369Prasanna NGayam PKRIbrahim RAranjani JMBiswas AfalseProduction, isolation, optimization, and characterization of microbial PHA from Bacillus australimaris.Population explosion in recent years has driven the environment to overuse nondegradable substances. Microbial polyesters known as polyhydroxyalkanoates (PHAs) are generated and retained as cytoplasmic granules in microorganisms with restricted nutritional availability and can be used to manufacture bioplastics. The current study attempts to screen soil isolates for PHA production and optimize their media parameters. Among all the isolates, 17 were identified and confirmed by Sudan black staining, as they are screening for PHA production and are identified by their colony characteristics. The isolation of the most promising strain, GS-14, was achieved through the sodium hypochlorite method, and subsequent quantification involved establishing a standard curve of crotonic acid. Notably, isol2025-01-01T00:00:00Z2025 Mar2025-04-04T09:07:47.379Z2025-04-04T09:07:47.379ZS-EPMC118973694006924610.1038/s41598-025-92146-x