biostudies-literatureSingh RNIBIB NIH HHSGeorge Mason UniversityDivision of Materials ResearchNational Institute of Biomedical Imaging and Bioengineering1445-1455https://www.ebi.ac.uk/biostudies/studies/S-EPMC11897940biostudies-literatureUnknown11(3)This study explores the synergistic effects of combining titania nanotubes (TiNTs) with the biopolymer Tanfloc (TAN) to enhance the surface properties of TiNTs for biomedical applications. We investigated the interactions of blood components and human adipose-derived stem cells (ADSCs) with TiNT surfaces covalently functionalized with Tanfloc (TAN), an aminolyzed polyphenolic tannin derivative. The functionalized surfaces (TiNT-TAN) have great potential to control protein adsorption and platelet adhesion and activation. Fluorescence and scanning electron microscopy (SEM) were used to analyze platelet adherence and activation. The amphoteric nature and multiple functional groups on TAN can control blood protein adsorption, platelet adhesion, and activation. Further, the modified surface supACS biomaterials science & engineeringTanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility.PMC118979402306983R21 EB0335111R21EB033511Martins AFMadruga LYCKipper MJPopat KCSavargaonkar ASingh RfalseTanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility.This study explores the synergistic effects of combining titania nanotubes (TiNTs) with the biopolymer Tanfloc (TAN) to enhance the surface properties of TiNTs for biomedical applications. We investigated the interactions of blood components and human adipose-derived stem cells (ADSCs) with TiNT surfaces covalently functionalized with Tanfloc (TAN), an aminolyzed polyphenolic tannin derivative. The functionalized surfaces (TiNT-TAN) have great potential to control protein adsorption and platelet adhesion and activation. Fluorescence and scanning electron microscopy (SEM) were used to analyze platelet adherence and activation. The amphoteric nature and multiple functional groups on TAN can control blood protein adsorption, platelet adhesion, and activation. Further, the modified surface sup2025-01-01T00:00:00Z2025 Mar2025-04-04T09:07:20.353Z2025-04-04T09:07:20.353ZS-EPMC118979404001366410.1021/acsbiomaterials.4c02106