biostudies-literaturevan den Dijssel JUniversity of MelbourneAustralian Research CouncilSanquin Blood Supply Project Grante202451565https://www.ebi.ac.uk/biostudies/studies/S-EPMC11898545biostudies-literatureUnknown55(3)Immunosenescence, age-related immune dysregulation, reduces immunity upon vaccinations and infections. Cytomegalovirus (CMV) infection results in declining naïve (T<sub>naïve</sub>) and increasing terminally differentiated (T<sub>emra</sub>) T cell populations, further aggravating immune aging. Both immunosenescence and CMV have been speculated to hamper the formation of protective T-cell immunity against novel or emerging pathogens. The SARS-CoV-2 pandemic presented a unique opportunity to examine the impact of age and/or CMV on the generation of de novo SARS-CoV-2-specific CD8<sup>+</sup> T cell responses in 40 younger (22-40 years) and 37 older (50-66 years) convalescent individuals. Heterotetramer combinatorial coding combined with phenotypic markers were used to study 35 SARS-CoV-2 epEuropean journal of immunologyAge and Latent Cytomegalovirus Infection Do Not Affect the Magnitude of De Novo SARS-CoV-2-Specific CD8<sup>+</sup> T Cell Responses.PMC11898545PPOC / L2506EstablishmentGrantARC-DECRA Fellowship / DE220100185van de Sandt CERaaphorst HTurksma AWVeldhuisen Bvan den Dijssel JKoets LTen Brinke Avan Lier RAWvan Ham SMde Jongh RFreen-van Heeren JJSteenhuis MKonijn VALDuurland MCvan der Schoot CEvan Gisbergen KPJMRispens TfalseAge and Latent Cytomegalovirus Infection Do Not Affect the Magnitude of De Novo SARS-CoV-2-Specific CD8<sup>+</sup> T Cell Responses.Immunosenescence, age-related immune dysregulation, reduces immunity upon vaccinations and infections. Cytomegalovirus (CMV) infection results in declining naïve (T<sub>naïve</sub>) and increasing terminally differentiated (T<sub>emra</sub>) T cell populations, further aggravating immune aging. Both immunosenescence and CMV have been speculated to hamper the formation of protective T-cell immunity against novel or emerging pathogens. The SARS-CoV-2 pandemic presented a unique opportunity to examine the impact of age and/or CMV on the generation of de novo SARS-CoV-2-specific CD8<sup>+</sup> T cell responses in 40 younger (22-40 years) and 37 older (50-66 years) convalescent individuals. Heterotetramer combinatorial coding combined with phenotypic markers were used to study 35 SARS-CoV-2 ep2025-01-01T00:00:00Z2025 Mar2025-04-04T09:07:36.449Z2025-04-04T09:07:36.449ZS-EPMC118985454007171110.1002/eji.202451565