biostudies-literaturePeng FDeutsche Forschungsgemeinschaft (DFG)Deutsche Forschungsgemeinschafte0285924https://www.ebi.ac.uk/biostudies/studies/S-EPMC11898615biostudies-literatureUnknown16(3)Structural maintenance of chromosomes (SMC) are ubiquitously distributed proteins involved in chromosome organization. Deletion of <i>smc</i> causes severe growth phenotypes in many organisms. Surprisingly, <i>smc</i> can be deleted in <i>Corynebacterium glutamicum</i>, a member of the <i>Actinomycetota</i> phylum, without any apparent growth phenotype. SMC in <i>C. glutamicum</i> is loaded in a ParB-dependent fashion to the chromosome and functions in replichore cohesion. The unexpected absence of a growth phenotype in the <i>smc</i> mutant prompted us to screen for synthetic interactions within <i>C. glutamicum</i>. We generated a high-density Tn5 library from wild-type and <i>smc</i>-deleted <i>C. glutamicum</i> strains. Transposon sequencing data revealed that the DNA translocase FtsK mBioEarly onset of septal FtsK localization allows for efficient DNA segregation in SMC-deleted <i>Corynebacterium glutamicum</i> strains.PMC11898615INST 257/645-1BR2815/6-2Linder MRuckert-Reed CBramkamp MHaak MPeng FKalinowski JMeyer FWeiß MGiacomelli GfalseEarly onset of septal FtsK localization allows for efficient DNA segregation in SMC-deleted <i>Corynebacterium glutamicum</i> strains.Structural maintenance of chromosomes (SMC) are ubiquitously distributed proteins involved in chromosome organization. Deletion of <i>smc</i> causes severe growth phenotypes in many organisms. Surprisingly, <i>smc</i> can be deleted in <i>Corynebacterium glutamicum</i>, a member of the <i>Actinomycetota</i> phylum, without any apparent growth phenotype. SMC in <i>C. glutamicum</i> is loaded in a ParB-dependent fashion to the chromosome and functions in replichore cohesion. The unexpected absence of a growth phenotype in the <i>smc</i> mutant prompted us to screen for synthetic interactions within <i>C. glutamicum</i>. We generated a high-density Tn5 library from wild-type and <i>smc</i>-deleted <i>C. glutamicum</i> strains. Transposon sequencing data revealed that the DNA translocase FtsK 2025-01-01T00:00:00Z2025 Mar2025-04-03T23:57:36.823Z2025-04-03T23:57:36.823ZS-EPMC118986153987348510.1128/mbio.02859-24