{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Almaqwashi AA"],"funding":["National Science Foundation","National Science Foundation Directorate for Biological Sciences"],"pagination":["667-676"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11900151"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["124(4)"],"pubmed_abstract":["Binuclear ruthenium complexes have been investigated for potential DNA-targeted therapeutic and diagnostic applications. Studies of DNA threading intercalation, in which DNA basepairs must be broken for intercalation, have revealed means of optimizing a model binuclear ruthenium complex to obtain reversible DNA-ligand assemblies with the desired properties of high affinity and slow kinetics. Here, we used single-molecule force spectroscopy to study a binuclear ruthenium complex with a longer semirigid linker relative to the model complex. Equilibrium results suggest a DNA affinity that is an order of magnitude higher than the parent binuclear ruthenium complex, likely due to a sterically relieved DNA threading intercalation mechanism. Notably, kinetics analysis shows that less DNA elongati"],"journal":["Biophysical journal"],"pubmed_title":["Binuclear ruthenium complex linker length tunes DNA threading intercalation kinetics."],"pmcid":["PMC11900151"],"funding_grant_id":["MCB-1817712"],"pubmed_authors":["Rouzina I","Andersson J","Almaqwashi AA","Westerlund F","Lincoln P","McCauley MJ","Williams MC"],"additional_accession":[]},"is_claimable":false,"name":"Binuclear ruthenium complex linker length tunes DNA threading intercalation kinetics.","description":"Binuclear ruthenium complexes have been investigated for potential DNA-targeted therapeutic and diagnostic applications. Studies of DNA threading intercalation, in which DNA basepairs must be broken for intercalation, have revealed means of optimizing a model binuclear ruthenium complex to obtain reversible DNA-ligand assemblies with the desired properties of high affinity and slow kinetics. Here, we used single-molecule force spectroscopy to study a binuclear ruthenium complex with a longer semirigid linker relative to the model complex. Equilibrium results suggest a DNA affinity that is an order of magnitude higher than the parent binuclear ruthenium complex, likely due to a sterically relieved DNA threading intercalation mechanism. Notably, kinetics analysis shows that less DNA elongati","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-07-16T05:07:02.062Z","creation":"2026-07-10T03:08:33.881Z"},"accession":"S-EPMC11900151","cross_references":{"pubmed":["39797403"],"doi":["10.1016/j.bpj.2025.01.002"]}}