biostudies-literatureXu JDouble First Class Construction Fund ProjectInner Mongolia Agricultural University Biopesticide Creation and Utilization Team829https://www.ebi.ac.uk/biostudies/studies/S-EPMC11902167biostudies-literatureUnknown14(5)China is the largest strawberry producer in the world. Strawberry black root rot is a novel disease that occurs in Hohhot, Inner Mongolia. In the present study, the inhibitory effects of <i>Bacillus subtilis</i> S-16 and its fermented form on strawberry black root rot caused by <i>Fusarium asiaticum</i> were tested. The inhibition rates were 56.31% and 65.95%, respectively. Furthermore, the metabolic substances were analysed using LC-MS/MS. A total of 68 substances were identified, including 18 amino acids, 7 of which have been reported to have pro-growth and antibacterial functions. Among these seven amino acids, N-acetyl-D-alloisoleucine (NAD) had the strongest inhibitory effect on <i>F. asiaticum</i>. In addition, NAD caused the mycelia of <i>F. asiaticum</i> to appear shrivelled and dePlants (Basel, Switzerland)Mechanism of N-Acetyl-D-alloisoleucine in Controlling Strawberry Black Root Rot.PMC11902167NDYB2023-48.BR22-13-11Niu RZhao MLi YHao JZhao SLi SZhang XZhang SWang ZZhou HXu JfalseMechanism of N-Acetyl-D-alloisoleucine in Controlling Strawberry Black Root Rot.China is the largest strawberry producer in the world. Strawberry black root rot is a novel disease that occurs in Hohhot, Inner Mongolia. In the present study, the inhibitory effects of <i>Bacillus subtilis</i> S-16 and its fermented form on strawberry black root rot caused by <i>Fusarium asiaticum</i> were tested. The inhibition rates were 56.31% and 65.95%, respectively. Furthermore, the metabolic substances were analysed using LC-MS/MS. A total of 68 substances were identified, including 18 amino acids, 7 of which have been reported to have pro-growth and antibacterial functions. Among these seven amino acids, N-acetyl-D-alloisoleucine (NAD) had the strongest inhibitory effect on <i>F. asiaticum</i>. In addition, NAD caused the mycelia of <i>F. asiaticum</i> to appear shrivelled and de2025-01-01T00:00:00Z2025 Mar2025-04-03T23:56:57.091Z2025-04-03T23:56:57.091ZS-EPMC119021674009482610.3390/plants14050829