biostudies-literatureCosta CJCCR NIH HHSIntramural NIH HHSNIMHD NIH HHSNCI NIH HHSNIH HHS921https://www.ebi.ac.uk/biostudies/studies/S-EPMC11902232biostudies-literatureUnknown17(5)<h4>Background</h4>Chronic visceral hypersensitivity is associated with an overstressed pain response to noxious stimuli (hyperalgesia). Microbiota are active modulators of host biology and are implicated in the etiology of visceral hypersensitivity.<h4>Objectives</h4>we studied the association between the circulating mRNA transcriptome, the intensity of induced visceral pain (IVP), and variation in the oral microbiome among participants with and without baseline visceral hypersensitivity.<h4>Methods</h4>Transcriptomic profiles and microbial abundance were correlated with IVP intensity. Host mRNA and microbes associated with IVP were explored, linking variation in the microbiome to host RNA biology.<h4>Results</h4>259 OTUs were found to be associated with IVP through correlation to differential expression of 471 genes in molecular pathways related to inflammation and neural mechanisms, including Rho and PI3K/AKT pathways. The bacterial families Lachnospiraceae, Prevotellaceae, and Veillonellaceae showed the highest degree of association. Oral microbial profiles with reduced diversity were characteristic of participants with visceral hypersensitivity.<h4>Conclusions</h4>Our results suggest that the oral microbiome may be involved in systemic immune and inflammatory effects and play a role in nervous system and stem cell pathways. The interactions between visceral hypersensitivity, differentially expressed molecular pathways, and microbiota described here provide a framework for further work exploring the relationship between host and microbiome.NutrientsHost Transcriptome and Microbial Variation in Relation to Visceral Hyperalgesia.PMC119022321ZIANR000018-01-09; K22MD006143-01K22 MD006143HHSN261200800001EZIA NR000018HHSN261200800001CPosada-Quintero HAbey SKHoffman RKFourie NHSherwin LBRahim-Williams BHenderson WACosta CJPrescott SJoseph PVfalseHost Transcriptome and Microbial Variation in Relation to Visceral Hyperalgesia.<h4>Background</h4>Chronic visceral hypersensitivity is associated with an overstressed pain response to noxious stimuli (hyperalgesia). Microbiota are active modulators of host biology and are implicated in the etiology of visceral hypersensitivity.<h4>Objectives</h4>we studied the association between the circulating mRNA transcriptome, the intensity of induced visceral pain (IVP), and variation in the oral microbiome among participants with and without baseline visceral hypersensitivity.<h4>Methods</h4>Transcriptomic profiles and microbial abundance were correlated with IVP intensity. Host mRNA and microbes associated with IVP were explored, linking variation in the microbiome to host RNA biology.<h4>Results</h4>259 OTUs were found to be associated with IVP through correlation to differential expression of 471 genes in molecular pathways related to inflammation and neural mechanisms, including Rho and PI3K/AKT pathways. The bacterial families Lachnospiraceae, Prevotellaceae, and Veillonellaceae showed the highest degree of association. Oral microbial profiles with reduced diversity were characteristic of participants with visceral hypersensitivity.<h4>Conclusions</h4>Our results suggest that the oral microbiome may be involved in systemic immune and inflammatory effects and play a role in nervous system and stem cell pathways. The interactions between visceral hypersensitivity, differentially expressed molecular pathways, and microbiota described here provide a framework for further work exploring the relationship between host and microbiome.2025-01-01T00:00:00Z2025 Mar2026-05-28T03:09:25.769Z2025-04-03T23:57:20.882ZS-EPMC119022324007779210.3390/nu17050921