biostudies-literatureRicardo JIBEROS+630https://www.ebi.ac.uk/biostudies/studies/S-EPMC11902790biostudies-literatureUnknown17(5)This research presents a novel, cost-effective, and scalable approach for the direct detection of myoglobin (Myo) in point-of-care (PoC) applications. In this strategy, redox-active Prussian Blue nanocubes (PBNCs) are applied to a disposable platinum screen-printed electrode (Pt-SPE). Subsequently, a biomimetic sensing layer is generated by electropolymerization of ortho-phenylenediamine (o-PD) in the presence of Myo, which forms molecularly imprinted polymer (MIP) sites by cyclic voltammetry (CV). The electropolymerization process takes place in a potential range of -0.2 V to +0.8 V, for five cycles at a scan rate of 50 mV/s, in a 10 mmol/L o-PD solution. After polymerization, the electrode is incubated in trypsin for 2 h to create Myo-specifically imprinted cavities. The structural and mPolymersBiomimetic Prussian Blue Sensor for Ultrasensitive Direct Detection of Myoglobin.PMC11902790(0072_IBEROS_MAIS_1_E, Interreg-POCTEP 2021-2027Moreira FTCRicardo JChiussi SMartins GVDuarte AfalseBiomimetic Prussian Blue Sensor for Ultrasensitive Direct Detection of Myoglobin.This research presents a novel, cost-effective, and scalable approach for the direct detection of myoglobin (Myo) in point-of-care (PoC) applications. In this strategy, redox-active Prussian Blue nanocubes (PBNCs) are applied to a disposable platinum screen-printed electrode (Pt-SPE). Subsequently, a biomimetic sensing layer is generated by electropolymerization of ortho-phenylenediamine (o-PD) in the presence of Myo, which forms molecularly imprinted polymer (MIP) sites by cyclic voltammetry (CV). The electropolymerization process takes place in a potential range of -0.2 V to +0.8 V, for five cycles at a scan rate of 50 mV/s, in a 10 mmol/L o-PD solution. After polymerization, the electrode is incubated in trypsin for 2 h to create Myo-specifically imprinted cavities. The structural and m2025-01-01T00:00:00Z2025 Feb2025-04-04T09:07:43.852Z2025-04-04T09:07:43.852ZS-EPMC119027904007612210.3390/polym17050630