{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Smith-Davidson P"],"funding":["National Center for Advancing Translational Sciences","CCR NIH HHS","Intramural NIH HHS","NCATS NIH HHS","National Heart, Lung, and Blood Institute","NIDCD NIH HHS","NHLBI NIH HHS","National Institute on Deafness and Other Communication Disorders","NCI NIH HHS","Center for Cancer Research"],"pagination":["S1-S8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11903372"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["135 Suppl 1"],"pubmed_abstract":["<h4>Objectives</h4>Allergic fungal rhinosinusitis (AFRS) is an eosinophilic subtype of chronic rhinosinusitis with nasal polyposis (CRSwNP). This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.<h4>Methods</h4>Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air/liquid interface and subjected to RNA-seq analysis, RT-qPCR, immunoblotting, and immunostaining.<h4>Results</h4>A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted p-value, was prostaglandin E receptor 2 (PTGER2). The upregulation of PTGER2 was confirmed by RT-qPCR and immunoblot. The presence of the EP2 receptor, encoded by the PTGER2 gene, was confirmed by immunocytochemistry.<h4>Conclusion</h4>PTGER2 is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin-exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.<h4>Level of evidence</h4>NA Laryngoscope, 135:S1-S8, 2025."],"journal":["The Laryngoscope"],"pubmed_title":["Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium."],"pmcid":["PMC11903372"],"funding_grant_id":["R03TR004022","F32 DC000097","UL1 TR002378","P30 CA138292","UL1TR002378","R01‐HL158979","DC000097","P30CA138292","R01-HL158979","R03 TR004022","R01 HL158979","ZIA DC000098"],"pubmed_authors":["Kabongo MM","DelGaudio JM","Koval M","Magliocca KR","Altartoor K","Solares CA","Claussen H","Levy JM","Johnston HR","Wise SK","Barrow EM","Smith-Davidson P","Arthur RA"],"additional_accession":[]},"is_claimable":false,"name":"Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.","description":"<h4>Objectives</h4>Allergic fungal rhinosinusitis (AFRS) is an eosinophilic subtype of chronic rhinosinusitis with nasal polyposis (CRSwNP). This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.<h4>Methods</h4>Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air/liquid interface and subjected to RNA-seq analysis, RT-qPCR, immunoblotting, and immunostaining.<h4>Results</h4>A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted p-value, was prostaglandin E receptor 2 (PTGER2). The upregulation of PTGER2 was confirmed by RT-qPCR and immunoblot. The presence of the EP2 receptor, encoded by the PTGER2 gene, was confirmed by immunocytochemistry.<h4>Conclusion</h4>PTGER2 is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin-exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.<h4>Level of evidence</h4>NA Laryngoscope, 135:S1-S8, 2025.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-06-02T21:05:13.028Z","creation":"2025-04-03T23:56:44.631Z"},"accession":"S-EPMC11903372","cross_references":{"pubmed":["39487665"],"doi":["10.1002/lary.31868"]}}