biostudies-literatureSmith-Davidson PNational Center for Advancing Translational SciencesCCR NIH HHSIntramural NIH HHSNCATS NIH HHSNational Heart, Lung, and Blood InstituteNIDCD NIH HHSNHLBI NIH HHSNational Institute on Deafness and Other Communication DisordersNCI NIH HHSCenter for Cancer ResearchS1-S8https://www.ebi.ac.uk/biostudies/studies/S-EPMC11903372biostudies-literatureUnknown135 Suppl 1<h4>Objectives</h4>Allergic fungal rhinosinusitis (AFRS) is an eosinophilic subtype of chronic rhinosinusitis with nasal polyposis (CRSwNP). This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.<h4>Methods</h4>Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air/liquid interface and subjected to RNA-seq analysis, RT-qPCR, immunoblotting, and immunostaining.<h4>Results</h4>A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted p-value, was prostaglandin E receptor 2 (PTGER2). The upregulation of PTGER2 was confirmed by RT-qPCR and immunoblot. The presence of the EP2 receptor, encoded by the PTGER2 gene, was confirmed by immunocytochemistry.<h4>Conclusion</h4>PTGER2 is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin-exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.<h4>Level of evidence</h4>NA Laryngoscope, 135:S1-S8, 2025.The LaryngoscopeProstaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.PMC11903372R03TR004022F32 DC000097UL1 TR002378P30 CA138292UL1TR002378R01‐HL158979DC000097P30CA138292R01-HL158979R03 TR004022R01 HL158979ZIA DC000098Kabongo MMDelGaudio JMKoval MMagliocca KRAltartoor KSolares CAClaussen HLevy JMJohnston HRWise SKBarrow EMSmith-Davidson PArthur RAfalseProstaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.<h4>Objectives</h4>Allergic fungal rhinosinusitis (AFRS) is an eosinophilic subtype of chronic rhinosinusitis with nasal polyposis (CRSwNP). This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.<h4>Methods</h4>Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air/liquid interface and subjected to RNA-seq analysis, RT-qPCR, immunoblotting, and immunostaining.<h4>Results</h4>A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted p-value, was prostaglandin E receptor 2 (PTGER2). The upregulation of PTGER2 was confirmed by RT-qPCR and immunoblot. The presence of the EP2 receptor, encoded by the PTGER2 gene, was confirmed by immunocytochemistry.<h4>Conclusion</h4>PTGER2 is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin-exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.<h4>Level of evidence</h4>NA Laryngoscope, 135:S1-S8, 2025.2025-01-01T00:00:00Z2025 Apr2026-06-02T21:05:13.028Z2025-04-03T23:56:44.631ZS-EPMC119033723948766510.1002/lary.31868