biostudies-literatureUnknown16Cui BThe fruits of <i>Lycium ruthenicum</i> Murr. (<i>Solanaceae</i>) are employed in ethnomedicine and used as a functional food. Their antioxidant, anti-aging, and hypolipidemic activities have been investigated in modern research. This study indicated that the ethanolic extract of the fruits of <i>L. ruthenicum</i> Murr. (LRM) improved oxidative and heat stress tolerance, reduced the accumulation of lipofuscin, and retarded the aging process in <i>Caenorhabditis elegans</i> (<i>Rhabditidae</i>). Furthermore, the pharyngeal pumping rate and body length decreased under LRM treatment. Moreover, metabolomic analysis and the DPClusO algorithm revealed that LRM regulated a series of lifespan-related pathways centered on glycine, serine, and threonine metabolism. These results suggest that LRM prolongs the lifespan of <i>Caenorhabditis elegans</i> via dietary restriction. Moreover, feruloyl putrescine, a kind of polyamine, was found in differential metabolites, which may be the metabolite of caffeoyl-spermidine in LRM. These findings from this exploratory study offer a new insight into the roles of <i>L. ruthenicum</i> in anti-aging activity as a functional food.Frontiers in pharmacology1498280https://www.ebi.ac.uk/biostudies/studies/S-EPMC11903438biostudies-literatureAnti-aging activities of an ethanolic extract of <i>Lycium ruthenicum</i> in <i>Caenorhabditis elegans</i> based on metabonomic analysis.PMC11903438Shan YQiao XLiu LFeng XXu SShi TYin MCui BfalseAnti-aging activities of an ethanolic extract of <i>Lycium ruthenicum</i> in <i>Caenorhabditis elegans</i> based on metabonomic analysis.The fruits of <i>Lycium ruthenicum</i> Murr. (<i>Solanaceae</i>) are employed in ethnomedicine and used as a functional food. Their antioxidant, anti-aging, and hypolipidemic activities have been investigated in modern research. This study indicated that the ethanolic extract of the fruits of <i>L. ruthenicum</i> Murr. (LRM) improved oxidative and heat stress tolerance, reduced the accumulation of lipofuscin, and retarded the aging process in <i>Caenorhabditis elegans</i> (<i>Rhabditidae</i>). Furthermore, the pharyngeal pumping rate and body length decreased under LRM treatment. Moreover, metabolomic analysis and the DPClusO algorithm revealed that LRM regulated a series of lifespan-related pathways centered on glycine, serine, and threonine metabolism. These results suggest that LRM prolongs the lifespan of <i>Caenorhabditis elegans</i> via dietary restriction. Moreover, feruloyl putrescine, a kind of polyamine, was found in differential metabolites, which may be the metabolite of caffeoyl-spermidine in LRM. These findings from this exploratory study offer a new insight into the roles of <i>L. ruthenicum</i> in anti-aging activity as a functional food.2025-01-01T00:00:00Z20252025-04-03T23:26:09.682Z2025-04-03T23:26:09.682ZS-EPMC119034384008338510.3389/fphar.2025.1498280