biostudies-literatureUnknown9(1)Siatka TMH-CZ-DROHumans must obtain vitamin B₉ (folate) from plant-based diet. The sources as well as the effect of food processing are discussed in detail. Industrial production, fortification and biofortification, kinetics, and physiological role in humans are described. As folate deficiency leads to several pathological states, current opinions toward prevention through fortification are discussed. Claimed risks of increased folate intake are mentioned as well as analytical ways for measurement of folate.NPJ science of food30https://www.ebi.ac.uk/biostudies/studies/S-EPMC11904035biostudies-literatureBiological, dietetic and pharmacological properties of vitamin B<sub>9</sub>.PMC11904035Mladenka PSuwanvecho CHarcarova PMatousova KMoravcova MMat'us MKrcmova LKSiatka TLomozova ZfalseBiological, dietetic and pharmacological properties of vitamin B<sub>9</sub>.Humans must obtain vitamin B₉ (folate) from plant-based diet. The sources as well as the effect of food processing are discussed in detail. Industrial production, fortification and biofortification, kinetics, and physiological role in humans are described. As folate deficiency leads to several pathological states, current opinions toward prevention through fortification are discussed. Claimed risks of increased folate intake are mentioned as well as analytical ways for measurement of folate.2025-01-01T00:00:00Z2025 Mar2025-04-26T05:53:46.902Z2025-04-06T11:38:55.207ZS-EPMC119040354007508110.1038/s41538-025-00396-w