biostudies-literatureZhao YNatural Science Foundation of Shanxi ProvinceState Key Laboratory for Mechanical Behavior of MaterialsFour "Batches" Innovation Project of Invigorating Medical through Science and Technology of Shanxi Provincee2409809https://www.ebi.ac.uk/biostudies/studies/S-EPMC11905086biostudies-literatureUnknown12(10)The interaction between biomaterials and immune system is a critical area of research, especially in tissue engineering and regenerative medicine. A fascinating and less explored aspect involves the immunomodulatory behaviors of macrophage (MΦ)-derived exosomes induced by biomaterial surfaces. Herein, untreated surface, nanostructured surface, and type I collagen (Col-I)-decorated nanostructured surface of titanium implants are chosen to culture MΦs, followed by extraction of MΦ-derived exosomes and investigation of their immunomodulatory functions and mechanisms. The results show that the exosomes in the untreated group carried plenty of inflammatory cytokines, predominantly C─C motif chemokine ligand 2 (CCL2). After targeting recipient cells, the CCL2 on the exosomes can specifically binAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Biomaterial Surface-Mediated Macrophages Exert Immunomodulatory Roles by Exosomal CCL2-Induced Membrane Integrin β1 Trafficking in Recipient Cells.PMC119050862022030212111732022XM1220232503Li HHang RHuang DXiao YYao RHan YWang HYao XSun YZhao YHuang XZhang XWang XfalseBiomaterial Surface-Mediated Macrophages Exert Immunomodulatory Roles by Exosomal CCL2-Induced Membrane Integrin β1 Trafficking in Recipient Cells.The interaction between biomaterials and immune system is a critical area of research, especially in tissue engineering and regenerative medicine. A fascinating and less explored aspect involves the immunomodulatory behaviors of macrophage (MΦ)-derived exosomes induced by biomaterial surfaces. Herein, untreated surface, nanostructured surface, and type I collagen (Col-I)-decorated nanostructured surface of titanium implants are chosen to culture MΦs, followed by extraction of MΦ-derived exosomes and investigation of their immunomodulatory functions and mechanisms. The results show that the exosomes in the untreated group carried plenty of inflammatory cytokines, predominantly C─C motif chemokine ligand 2 (CCL2). After targeting recipient cells, the CCL2 on the exosomes can specifically bin2025-01-01T00:00:00Z2025 Mar2025-04-04T03:04:18.308Z2025-04-04T03:04:18.308ZS-EPMC119050863983648810.1002/advs.202409809