{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moghe P"],"funding":["European Research Council"],"pagination":["408-423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11906357"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(3)"],"pubmed_abstract":["Tissue patterning coordinates morphogenesis, cell dynamics and fate specification. Understanding how precision in patterning is robustly achieved despite inherent developmental variability during mammalian embryogenesis remains a challenge. Here, based on cell dynamics quantification and simulation, we show how salt-and-pepper epiblast and primitive endoderm (PrE) cells pattern the inner cell mass of mouse blastocysts. Coupling cell fate and dynamics, PrE cells form apical polarity-dependent actin protrusions required for RAC1-dependent migration towards the surface of the fluid cavity, where PrE cells are trapped due to decreased tension. Concomitantly, PrE cells deposit an extracellular matrix gradient, presumably breaking the tissue-level symmetry and collectively guiding their own migration. Tissue size perturbations of mouse embryos and their comparison with monkey and human blastocysts further demonstrate that the fixed proportion of PrE/epiblast cells is optimal with respect to embryo size and tissue geometry and, despite variability, ensures patterning robustness during early mammalian development."],"journal":["Nature cell biology"],"pubmed_title":["Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness."],"pmcid":["PMC11906357"],"funding_grant_id":["742732"],"pubmed_authors":["Erzberger A","Hiiragi T","Iwatani C","Moghe P","Ichikawa T","Tsukiyama T","Belousov R"],"additional_accession":[]},"is_claimable":false,"name":"Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness.","description":"Tissue patterning coordinates morphogenesis, cell dynamics and fate specification. Understanding how precision in patterning is robustly achieved despite inherent developmental variability during mammalian embryogenesis remains a challenge. Here, based on cell dynamics quantification and simulation, we show how salt-and-pepper epiblast and primitive endoderm (PrE) cells pattern the inner cell mass of mouse blastocysts. Coupling cell fate and dynamics, PrE cells form apical polarity-dependent actin protrusions required for RAC1-dependent migration towards the surface of the fluid cavity, where PrE cells are trapped due to decreased tension. Concomitantly, PrE cells deposit an extracellular matrix gradient, presumably breaking the tissue-level symmetry and collectively guiding their own migration. Tissue size perturbations of mouse embryos and their comparison with monkey and human blastocysts further demonstrate that the fixed proportion of PrE/epiblast cells is optimal with respect to embryo size and tissue geometry and, despite variability, ensures patterning robustness during early mammalian development.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-04-22T07:26:19.208Z","creation":"2025-04-05T22:07:21.558Z"},"accession":"S-EPMC11906357","cross_references":{"pubmed":["39966670"],"doi":["10.1038/s41556-025-01618-9"]}}