biostudies-literatureUnknown16Guan Q<h4>Introduction</h4>Pyroptosis is an important inflammatory form of cell death and <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>) chronic infection triggers excessive inflammatory pyroptosis of macrophages. Our previous research has confirmed that a small compound pyrvinium pamoate (PP) could inhibit inflammatory pathological changes and mycobacterial burden in <i>M.tb</i>-infected mice, but the potential mechanism of PP for inhibiting <i>M.tb</i>-induced inflammation remains unexplored.<h4>Methods</h4>The effects of PP on the NLRP3-ASC-Casp1 inflammasome assembly and activation, gasdermin D (GSDMD) mediated pyroptosis and inflammatory cytokines expression were assessed in human THP-1-derived macrophages after <i>M.tb</i> H37Rv/H37Ra/ <i>Salmonella typhimurium</i> (<i>S. typhimurium</i>)Frontiers in immunology1553093https://www.ebi.ac.uk/biostudies/studies/S-EPMC11906677biostudies-literatureSuppression of NLRP3 inflammasome by a small molecule targeting CK1α-β-catenin-NF-κB and CK1α-NRF2-mitochondrial OXPHOS pathways during mycobacterial infection.PMC11906677Xie YNie QGuan QLiu SSong XZhang XLGuang YXiong HfalseSuppression of NLRP3 inflammasome by a small molecule targeting CK1α-β-catenin-NF-κB and CK1α-NRF2-mitochondrial OXPHOS pathways during mycobacterial infection.<h4>Introduction</h4>Pyroptosis is an important inflammatory form of cell death and <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>) chronic infection triggers excessive inflammatory pyroptosis of macrophages. Our previous research has confirmed that a small compound pyrvinium pamoate (PP) could inhibit inflammatory pathological changes and mycobacterial burden in <i>M.tb</i>-infected mice, but the potential mechanism of PP for inhibiting <i>M.tb</i>-induced inflammation remains unexplored.<h4>Methods</h4>The effects of PP on the NLRP3-ASC-Casp1 inflammasome assembly and activation, gasdermin D (GSDMD) mediated pyroptosis and inflammatory cytokines expression were assessed in human THP-1-derived macrophages after <i>M.tb</i> H37Rv/H37Ra/ <i>Salmonella typhimurium</i> (<i>S. typhimurium</i>)2025-01-01T00:00:00Z20252025-04-20T00:08:06.737Z2025-04-20T00:08:06.737ZS-EPMC119066774009299110.3389/fimmu.2025.1553093