{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jager S"],"funding":["Dutch Research Council (NWO)"],"pagination":["2497"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11906852"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Recently, a conceptually new mass analyzer was introduced by pairing a quadrupole Orbitrap mass spectrometer with an asymmetric track lossless (Astral™) analyzer. This system provides >200 Hz MS/MS scanning speed, high resolving power, sensitivity, and mass accuracy. Due to its speed, the instrument allows for a narrow-window data-independent acquisition (nDIA) strategy, representing a new technical milestone in peptide-centric proteomics. However, this new system may also be applied to other complex and clinically important proteomes, such as the human plasma N-glycoproteome. Here, we evaluate the Orbitrap Astral mass spectrometer for the in-depth analysis of the plasma N-glycoproteome and pioneer a dedicated nDIA workflow, termed \"nGlycoDIA\", on glycopeptide enriched and crude plasma. Th"],"journal":["Nature communications"],"pubmed_title":["In-depth plasma N-glycoproteome profiling using narrow-window data-independent acquisition on the Orbitrap Astral mass spectrometer."],"pmcid":["PMC11906852"],"funding_grant_id":["184.034.019","SPI.2017.028"],"pubmed_authors":["Heck AJR","Damoc E","Zeller M","Reiding KR","Makarov AA","Pashkova A","Jager S","Schulte D"],"additional_accession":[]},"is_claimable":false,"name":"In-depth plasma N-glycoproteome profiling using narrow-window data-independent acquisition on the Orbitrap Astral mass spectrometer.","description":"Recently, a conceptually new mass analyzer was introduced by pairing a quadrupole Orbitrap mass spectrometer with an asymmetric track lossless (Astral™) analyzer. This system provides >200 Hz MS/MS scanning speed, high resolving power, sensitivity, and mass accuracy. Due to its speed, the instrument allows for a narrow-window data-independent acquisition (nDIA) strategy, representing a new technical milestone in peptide-centric proteomics. However, this new system may also be applied to other complex and clinically important proteomes, such as the human plasma N-glycoproteome. Here, we evaluate the Orbitrap Astral mass spectrometer for the in-depth analysis of the plasma N-glycoproteome and pioneer a dedicated nDIA workflow, termed \"nGlycoDIA\", on glycopeptide enriched and crude plasma. Th","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-06-01T14:37:19.469Z","creation":"2025-04-04T00:06:34.004Z"},"accession":"S-EPMC11906852","cross_references":{"pubmed":["40082474"],"doi":["10.1038/s41467-025-57916-1"]}}