biostudies-literatureUnknown29Wei SCellular senescence, a process that induces irreversible cell cycle arrest in response to diverse stressors, is a primary contributor to aging and age-related diseases. Currently, exposure to hydrogen peroxide is a widely used technique for establishing in vitro cellular senescence models; however, this traditional method is inconsistent, laborious, and ineffective in vivo. To overcome these limitations, we have developed a hydrogen peroxide-releasing hydrogel that can readily and controllably induce senescence in conventional 2-dimensional cell cultures as well as advanced 3-dimensional microphysiological systems. Notably, we have established 2 platforms using our hydrogen peroxide-releasing hydrogel for investigating senolytics, which is a promising innovation in anti-geronic therapy. CoBiomaterials research0161https://www.ebi.ac.uk/biostudies/studies/S-EPMC11907071biostudies-literatureHydrogen Peroxide-Releasing Hydrogel-Mediated Cellular Senescence Model for Aging Research.PMC11907071Le Thi PHoang TTTJang YCDao TRyu DPark MYCho HKang YJZhang YDo KWei SOh CMHeo JMorgan NJo YPark KDfalseHydrogen Peroxide-Releasing Hydrogel-Mediated Cellular Senescence Model for Aging Research.Cellular senescence, a process that induces irreversible cell cycle arrest in response to diverse stressors, is a primary contributor to aging and age-related diseases. Currently, exposure to hydrogen peroxide is a widely used technique for establishing in vitro cellular senescence models; however, this traditional method is inconsistent, laborious, and ineffective in vivo. To overcome these limitations, we have developed a hydrogen peroxide-releasing hydrogel that can readily and controllably induce senescence in conventional 2-dimensional cell cultures as well as advanced 3-dimensional microphysiological systems. Notably, we have established 2 platforms using our hydrogen peroxide-releasing hydrogel for investigating senolytics, which is a promising innovation in anti-geronic therapy. Co2025-01-01T00:00:00Z20252025-04-22T07:27:09.256Z2025-04-05T22:08:58.391ZS-EPMC119070714009265110.34133/bmr.0161