biostudies-literatureFerguson DProstate Cancer UKEngineering and Physical Sciences Research Council1741-1753https://www.ebi.ac.uk/biostudies/studies/S-EPMC11907692biostudies-literatureUnknown150(9)One of the major limitations for clinical applications of infrared spectroscopic imaging modalities is the acquisition time required to obtain reasonable images of tissues with high spatial resolution and good signal-to-noise ratio (SNR). The time to acquire a reasonable signal to noise spectroscopic scan of a standard microscope slide region of tissue can take many hours. As a trade-off, systems can allow for discrete wavenumber acquisitions, sacrificing potentially vital chemical bands in order to reach specific acquisition targets. Recent instrumentation developments now allow for the full fingerprint imaging of entire microscope slides in under 30 minutes, enabling rapid, high quality spectroscopic imaging of tissues within clinical timeframes without sacrificing frequency bands. Here The AnalystFull fingerprint hyperspectral imaging of prostate cancer tissue microarrays within clinical timeframes using quantum cascade laser microscopy.PMC11907692EP/W00058X/1RIA21-ST2-011Hart CAOliveira PBrown MDreisbach DSachdeva AFerguson DGardner PClarke NKroeger-Lui NSanchez DFfalseFull fingerprint hyperspectral imaging of prostate cancer tissue microarrays within clinical timeframes using quantum cascade laser microscopy.One of the major limitations for clinical applications of infrared spectroscopic imaging modalities is the acquisition time required to obtain reasonable images of tissues with high spatial resolution and good signal-to-noise ratio (SNR). The time to acquire a reasonable signal to noise spectroscopic scan of a standard microscope slide region of tissue can take many hours. As a trade-off, systems can allow for discrete wavenumber acquisitions, sacrificing potentially vital chemical bands in order to reach specific acquisition targets. Recent instrumentation developments now allow for the full fingerprint imaging of entire microscope slides in under 30 minutes, enabling rapid, high quality spectroscopic imaging of tissues within clinical timeframes without sacrificing frequency bands. Here 2025-01-01T00:00:00Z2025 Apr2025-07-06T03:04:19.497Z2025-04-05T22:06:32.88ZS-EPMC119076924008456810.1039/d5an00046g