biostudies-literatureUnknown24(1)Opondo KOMinistry of Health and Sanitation, Sierra LeoneUnited States President’s Malaria Initiative through the United States Agency for International Development VectorLink ProjectUnited States President’s Malaria Initiative through the United States Agency for International Development Evolve Project<h4>Background</h4>Information on the status of insecticide resistance in malaria vectors is critical for implementing effective malaria vector control. The Sierra Leone National Malaria Control Programme, in collaboration with the PMI VectorLink project, assessed the resistance status to insecticides commonly used in public health, and associated resistance mechanisms in Anopheles gambiae, the main vector of malaria in Sierra Leone.<h4>Methods</h4>The susceptibility of An. gambiae against pyrethroids with and without piperonyl butoxide (PBO), chlorfenapyr, clothianidin, bendiocarb and pirimiphos-methyl was evaluated in four districts of Sierra Leone in 2018 and 2019 using WHO and CDC bottle bioassay protocols. A subset of samples that were exposed to the insecticides were screened for molMalaria journal80https://www.ebi.ac.uk/biostudies/studies/S-EPMC11907884biostudies-literatureCharacterization of insecticide resistance mechanisms in the Anopheles gambiae population of Sierra Leone.PMC11907884Conteh SLolleh UMokuena MOpondo KOYihdego YHughes TSchnabel DAlyko ELevine RJose RDonnelly JYamba FSmith SJalloh AJacob DIyikirenga LfalseCharacterization of insecticide resistance mechanisms in the Anopheles gambiae population of Sierra Leone.<h4>Background</h4>Information on the status of insecticide resistance in malaria vectors is critical for implementing effective malaria vector control. The Sierra Leone National Malaria Control Programme, in collaboration with the PMI VectorLink project, assessed the resistance status to insecticides commonly used in public health, and associated resistance mechanisms in Anopheles gambiae, the main vector of malaria in Sierra Leone.<h4>Methods</h4>The susceptibility of An. gambiae against pyrethroids with and without piperonyl butoxide (PBO), chlorfenapyr, clothianidin, bendiocarb and pirimiphos-methyl was evaluated in four districts of Sierra Leone in 2018 and 2019 using WHO and CDC bottle bioassay protocols. A subset of samples that were exposed to the insecticides were screened for mol2025-01-01T00:00:00Z2025 Mar2025-04-04T00:08:46.717Z2025-04-04T00:08:46.717ZS-EPMC119078844008289010.1186/s12936-025-05267-z