biostudies-literatureUnknown27Yu ZNational Natural Science Foundation of ChinaTandem repeat sequences (TRs), a class of repetitive genomic elements, are broadly distributed in both coding and non-coding regions. Investigating the relationship between sequences and function is essential for understanding the genome. <i>Saccharomyces cerevisiae</i> serves as a vital model organism and is widely used as an engineered strain. Although the transcriptional regulatory functions of TRs in the promoters of <i>S.cerevisiae</i> have been elucidated, our understanding of their roles within coding sequences (CDS) remains limited. In this study, we integrate RNA-seq, ChIP-seq, ATAC-seq, Hi-C, and Micro-C data from <i>S.cerevisiae</i> to analyze the types and distribution of TRs, and their impact on gene expression. Our results indicate that genes containing short tandem repeats (Computational and structural biotechnology journal705-716https://www.ebi.ac.uk/biostudies/studies/S-EPMC11908461biostudies-literatureIdentifying associations between short tandem repeat sequences and gene expression in yeast reveals specific repeated motifs encoding transcriptional regulatory proteins.PMC11908461Xu KRan DLiang YLuo YBo XChen HYu ZXu XXiang MChen BfalseIdentifying associations between short tandem repeat sequences and gene expression in yeast reveals specific repeated motifs encoding transcriptional regulatory proteins.Tandem repeat sequences (TRs), a class of repetitive genomic elements, are broadly distributed in both coding and non-coding regions. Investigating the relationship between sequences and function is essential for understanding the genome. <i>Saccharomyces cerevisiae</i> serves as a vital model organism and is widely used as an engineered strain. Although the transcriptional regulatory functions of TRs in the promoters of <i>S.cerevisiae</i> have been elucidated, our understanding of their roles within coding sequences (CDS) remains limited. In this study, we integrate RNA-seq, ChIP-seq, ATAC-seq, Hi-C, and Micro-C data from <i>S.cerevisiae</i> to analyze the types and distribution of TRs, and their impact on gene expression. Our results indicate that genes containing short tandem repeats (2025-01-01T00:00:00Z20252026-06-01T06:07:10.023Z2025-04-06T22:09:56.684ZS-EPMC119084614009266010.1016/j.csbj.2025.02.003