biostudies-literatureQian QScientific Research Projects of Medical and Health Institutions of Longhua District, ShenzhenShenzhen Science and Technology Program64https://www.ebi.ac.uk/biostudies/studies/S-EPMC11909235biostudies-literatureUnknown25(1)Ischemic stroke (IS), a leading cause of global disability and mortality, is characterized by white matter damage and demyelination. Despite advances, the molecular mechanisms driving post-IS myelin pathology remain poorly understood, limiting therapeutic development. This study investigates key myelin-related genes (MRGs) and their regulatory networks to identify novel therapeutic targets. A transient middle cerebral artery occlusion (MCAO) model was established in C57BL/6 mice, with brain tissues collected at four timepoints (Sham0D, MCAO0D, MCAO7D, MCAO14D). Transcriptomic and proteomic sequencing were performed, followed by soft clustering (Mfuzz), functional enrichment (GO/KEGG), and ROC analysis to identify key MRGs. Competing endogenous RNA (ceRNA) networks were constructed, and druFunctional & integrative genomicsCombined transcriptomic and proteomic analyses reveal relevant myelin features in mice with ischemic stroke.PMC119092352022073RCBS20210609104401012JCYJ20220530165207017Liu XShen MWang QHe YLi DLyu HWang WQian QfalseCombined transcriptomic and proteomic analyses reveal relevant myelin features in mice with ischemic stroke.Ischemic stroke (IS), a leading cause of global disability and mortality, is characterized by white matter damage and demyelination. Despite advances, the molecular mechanisms driving post-IS myelin pathology remain poorly understood, limiting therapeutic development. This study investigates key myelin-related genes (MRGs) and their regulatory networks to identify novel therapeutic targets. A transient middle cerebral artery occlusion (MCAO) model was established in C57BL/6 mice, with brain tissues collected at four timepoints (Sham0D, MCAO0D, MCAO7D, MCAO14D). Transcriptomic and proteomic sequencing were performed, followed by soft clustering (Mfuzz), functional enrichment (GO/KEGG), and ROC analysis to identify key MRGs. Competing endogenous RNA (ceRNA) networks were constructed, and dru2025-01-01T00:00:00Z2025 Mar2026-06-14T03:09:50.818Z2025-04-20T00:09:53.536ZS-EPMC119092354008534810.1007/s10142-025-01573-6