biostudies-literatureUnknown4(2)Liu Q<h4>Background</h4>Breast cancer has become a severe threat to human health, making it imperative to identify effective drugs and therapeutic targets.<h4>Methods</h4>Various molecular biology experiments, such as western blot analysis, cytologic effect, co-immunoprecipitation, and immunofluorescence assays, as well as a nude mouse xenograft tumor model, were used to comprehensively analyze the impact of gamma-interferon-inducible lysosomal thiol reductase (GILT) on the malignant phenotype of breast cancer cells. This work was performed to examine GILT expression levels and explore the potential mechanism in breast cancer.<h4>Results</h4>GILT protein expression levels were significantly lower in breast cancer cells than in normal breast epithelial cells. Overexpressing GILT inhibited breastCancer innovatione161https://www.ebi.ac.uk/biostudies/studies/S-EPMC11909800biostudies-literatureInvestigating the Mechanism of IFN-γ-Inducible Lysosomal Thiol Reductase-Mediated Inhibition of Breast Cancer Cell Proliferation.PMC11909800Liu HLi HShao YGuan XFeng KZhang JChen LTian YLiu QYuan XChu MWei LfalseInvestigating the Mechanism of IFN-γ-Inducible Lysosomal Thiol Reductase-Mediated Inhibition of Breast Cancer Cell Proliferation.<h4>Background</h4>Breast cancer has become a severe threat to human health, making it imperative to identify effective drugs and therapeutic targets.<h4>Methods</h4>Various molecular biology experiments, such as western blot analysis, cytologic effect, co-immunoprecipitation, and immunofluorescence assays, as well as a nude mouse xenograft tumor model, were used to comprehensively analyze the impact of gamma-interferon-inducible lysosomal thiol reductase (GILT) on the malignant phenotype of breast cancer cells. This work was performed to examine GILT expression levels and explore the potential mechanism in breast cancer.<h4>Results</h4>GILT protein expression levels were significantly lower in breast cancer cells than in normal breast epithelial cells. Overexpressing GILT inhibited breast2025-01-01T00:00:00Z2025 Apr2025-04-22T07:27:38.677Z2025-04-05T22:08:26.174ZS-EPMC119098004009407310.1002/cai2.161