biostudies-literatureUnknown26(1)Lehle SUniversitätsklinikum Erlangen<h4>Background</h4>Peripheral blood mononuclear cells (PBMCs) are valuable biomarkers, providing crucial insights into the patients' immune system. Reliable biobanking of PBMCs is essential to minimize heterogeneity. In multicenter trials, blood sample transportation to central laboratories can increase the time between blood collection and PBMC isolation. This study evaluated the effect of prolonged blood hold time on PBMC viability and cytotoxicity.<h4>Methods</h4>From July 2021 to May 2023, 104 patients with early HER2-positive breast cancer were enrolled in the NeoOn trial, of whom 49 patients were included in this subproject. PBMCs were isolated ≤ 6 hours (h) or ≥ 20 h after blood collection. PBMC yield and viability were determined using the LUNA-II Automated Cell Counter. Flow cytomBMC immunology21https://www.ebi.ac.uk/biostudies/studies/S-EPMC11909936biostudies-literatureEffect of delayed isolation of peripheral blood mononuclear cells on cell viability and functionality.PMC11909936Uhrig SVolkl SLehle SSeitz KEmons JTheuser AKBeckmann MWRuebner MZiegler PFasching PAGoossens CHuebner HfalseEffect of delayed isolation of peripheral blood mononuclear cells on cell viability and functionality.<h4>Background</h4>Peripheral blood mononuclear cells (PBMCs) are valuable biomarkers, providing crucial insights into the patients' immune system. Reliable biobanking of PBMCs is essential to minimize heterogeneity. In multicenter trials, blood sample transportation to central laboratories can increase the time between blood collection and PBMC isolation. This study evaluated the effect of prolonged blood hold time on PBMC viability and cytotoxicity.<h4>Methods</h4>From July 2021 to May 2023, 104 patients with early HER2-positive breast cancer were enrolled in the NeoOn trial, of whom 49 patients were included in this subproject. PBMCs were isolated ≤ 6 hours (h) or ≥ 20 h after blood collection. PBMC yield and viability were determined using the LUNA-II Automated Cell Counter. Flow cytom2025-01-01T00:00:00Z2025 Mar2026-06-01T19:05:55.255Z2025-04-04T00:31:50.901ZS-EPMC119099364008971410.1186/s12865-025-00701-y