{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He Q"],"funding":["Natural Science Foundation of Sichuan Province of China"],"pagination":["9186"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11914554"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["To compare the performance of magnetic bead (MB) and the concentrated precipitation (CP) based RT-qPCR to qualify cell free EBV DNA (cfEBV DNA) for nasopharyngeal carcinoma (NPC) in non-endemic area of China. From January 2014 to June 2024, a retrospective analysis of 2 cohort studies on cfEBV DNA in NPC patients was conducted to assess the diagnostic value, positive detection rate and clinical application. cfEBV DNA detection with CP based RT-qPCR in cohort 1 and MB based RT-qPCR method in cohort 2. The MB based RT-qPCR for the quantitative measurement of cfEBV DNA load was higher than the CP based RT-qPCR in the same plasma samples from NPC patients (P < 0.001). CP based RT-qPCR measured cfEBV DNA in 1405 NPC and 244 healthy controls in cohort 1 with 40.8% sensitivity (AUC = 0.704, 95% C"],"journal":["Scientific reports"],"pubmed_title":["MB based RT-qPCR increase the clinical application of cfEBV DNA for NPC in non-endemic area of China."],"pmcid":["PMC11914554"],"funding_grant_id":["23NSFSC1446"],"pubmed_authors":["Wang D","Yang M","Luo H","He Q","Zhou Y","Yan Y","Liu X","Meng F","Tuo Y","Ying B","Wang Q","Huang Y","Zhang Z","Zhao D"],"additional_accession":[]},"is_claimable":false,"name":"MB based RT-qPCR increase the clinical application of cfEBV DNA for NPC in non-endemic area of China.","description":"To compare the performance of magnetic bead (MB) and the concentrated precipitation (CP) based RT-qPCR to qualify cell free EBV DNA (cfEBV DNA) for nasopharyngeal carcinoma (NPC) in non-endemic area of China. From January 2014 to June 2024, a retrospective analysis of 2 cohort studies on cfEBV DNA in NPC patients was conducted to assess the diagnostic value, positive detection rate and clinical application. cfEBV DNA detection with CP based RT-qPCR in cohort 1 and MB based RT-qPCR method in cohort 2. The MB based RT-qPCR for the quantitative measurement of cfEBV DNA load was higher than the CP based RT-qPCR in the same plasma samples from NPC patients (P < 0.001). CP based RT-qPCR measured cfEBV DNA in 1405 NPC and 244 healthy controls in cohort 1 with 40.8% sensitivity (AUC = 0.704, 95% C","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-04-21T16:53:37.681Z","creation":"2025-04-21T16:53:37.681Z"},"accession":"S-EPMC11914554","cross_references":{"pubmed":["40097593"],"doi":["10.1038/s41598-025-93406-6"]}}