{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aggarwal T"],"funding":["American Cancer Society","Rutgers Cancer Institute of New Jersey","NIBIB NIH HHS","Steven A. Cox Foundation","NCI NIH HHS","National Institute of Biomedical Imaging and Bioengineering"],"pagination":["e202421936"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11914935"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["64(12)"],"pubmed_abstract":["Fluorescent light-up aptamer (FLAP) systems are promising (bio)sensing platforms that are genetically encodable. However, FLAP-mediated detection of each distinct target necessitates either in vitro selection or engineering of nucleic acid sequences. Furthermore, an aptamer that binds an inorganic target or a chemical species with a short lifetime is challenging to realize. Here, we describe a small-molecule approach that makes it possible for a single FLAP system to detect chemically unique, non-fluorogenic, and reactive inorganics. We developed functionalized pre-ligands of RNA aptamers that bind benzylidene imidazolinones (Baby Spinach, Broccolli, Squash). Reactive inorganics, hydrogen sulfide (H<sub>2</sub>S/HS<sup>-</sup>) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), can specif"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["A Small-Molecule Approach Enables RNA Aptamers to Function as Sensors for Reactive Inorganic Targets."],"pmcid":["PMC11914935"],"funding_grant_id":["R21 EB029548","P30 CA072720","P30CA072720","EB029548"],"pubmed_authors":["Aggarwal T","Erguven H","Guven H","Izgu EC","Gutierrez B","Cho S","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"A Small-Molecule Approach Enables RNA Aptamers to Function as Sensors for Reactive Inorganic Targets.","description":"Fluorescent light-up aptamer (FLAP) systems are promising (bio)sensing platforms that are genetically encodable. However, FLAP-mediated detection of each distinct target necessitates either in vitro selection or engineering of nucleic acid sequences. Furthermore, an aptamer that binds an inorganic target or a chemical species with a short lifetime is challenging to realize. Here, we describe a small-molecule approach that makes it possible for a single FLAP system to detect chemically unique, non-fluorogenic, and reactive inorganics. We developed functionalized pre-ligands of RNA aptamers that bind benzylidene imidazolinones (Baby Spinach, Broccolli, Squash). Reactive inorganics, hydrogen sulfide (H<sub>2</sub>S/HS<sup>-</sup>) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), can specif","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-06-01T19:01:14.408Z","creation":"2025-04-04T03:02:12.352Z"},"accession":"S-EPMC11914935","cross_references":{"pubmed":["39666858"],"doi":["10.1002/anie.202421936"]}}