{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Xiao N"],"funding":["Lo Ying Shek Chi Wai Foundation","Innovation and Technology Commission","the Hong Kong Hainan Commercial Association"],"pubmed_abstract":["The newly emerged avian influenza A H5N1 Clade 2.3.4.4b can infect dairy cows and shed live virus in their milk. Sporadic cattle-to-human infections have been reported, highlighting the urgent need to understand its pathogenesis in mammals. Using both non-lactating and lactating BALB/c mice, we examined the viral tissue tropism, histopathological damages, and host immune responses upon intranasal inoculation with a reverse-genetic virus constructed based on A/dairy cattle/Texas/24-008749-003/2024 (Cattle-H5N1) and comparing with an older reference Clade 1 virus, A/Vietnam/1194/2004 virus (VNM1194-H5N1). Cattle-H5N1 was highly lethal in mice (mLD<sub>50</sub> = 1.48PFU) with broad tissue tropism and produced higher titer in respiratory tissue and multiple extrapulmonary organs than VNM1194-"],"journal":["Emerging microbes & infections"],"pagination":["2475836"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11915741"],"repository":["biostudies-literature"],"pubmed_title":["Reverse genetics-derived cattle H5N1 virus from Clade 2.3.4.4b shows enhanced systemic infectivity and pathogenicity than an older Clade 1 H5N1 virus in BALB/c mice."],"pmcid":["PMC11915741"],"pubmed_authors":["Yuan S","Li C","Cai J","Chan JF","Zhang AJ","Chung HC","Chen H","Ye Z","Xiao N","Wang P","Lam AH","Chu H","Chen Y","Yuen KY","Oong XY","Lee AC","Song W","Kok KH"],"additional_accession":[]},"is_claimable":false,"name":"Reverse genetics-derived cattle H5N1 virus from Clade 2.3.4.4b shows enhanced systemic infectivity and pathogenicity than an older Clade 1 H5N1 virus in BALB/c mice.","description":"The newly emerged avian influenza A H5N1 Clade 2.3.4.4b can infect dairy cows and shed live virus in their milk. Sporadic cattle-to-human infections have been reported, highlighting the urgent need to understand its pathogenesis in mammals. Using both non-lactating and lactating BALB/c mice, we examined the viral tissue tropism, histopathological damages, and host immune responses upon intranasal inoculation with a reverse-genetic virus constructed based on A/dairy cattle/Texas/24-008749-003/2024 (Cattle-H5N1) and comparing with an older reference Clade 1 virus, A/Vietnam/1194/2004 virus (VNM1194-H5N1). Cattle-H5N1 was highly lethal in mice (mLD<sub>50</sub> = 1.48PFU) with broad tissue tropism and produced higher titer in respiratory tissue and multiple extrapulmonary organs than VNM1194-","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-04-08T19:52:35.649Z","creation":"2026-04-08T14:31:14.947Z"},"accession":"S-EPMC11915741","cross_references":{"pubmed":["40035774"],"doi":["10.1080/22221751.2025.2475836"]}}