biostudies-literatureMillar EVMilitary Infectious Disease Research ProgramNIAID NIH HHSU.S. Department of Defense729-737https://www.ebi.ac.uk/biostudies/studies/S-EPMC11915751biostudies-literatureUnknown47(5)<h4>Purpose</h4>Individuals with methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI) can be simultaneously colonized with MRSA on multiple body sites. Using whole genome sequencing (WGS), the intrahost relatedness of MRSA colonization and infection isolates was investigated.<h4>Methods</h4>In the context of a prospective case-control study of SSTI, we analyzed colonization and infection isolates from US Army Infantry trainees with purulent infection due to MRSA. At the time of clinical presentation for SSTI, culture swabs were obtained from the infection site, as well as from the patient's nasal, oral, inguinal, and perianal regions. S. aureus culture and susceptibility was performed by standard methods. DNA from MRSA isolates was extracted and librarieInfectionGenomic epidemiology of MRSA infection and colonization isolates among military trainees with skin and soft tissue infection.PMC11915751HT9404-12-1-0019HT9404-12-1-0012HU0001-10-1-0018Y01 AI005072Cer RZTribble DREllis MWSchlett CDBennett JWBishop-Lilly KARice GKHamilton TMillar EVElassal EMFrey KGfalseGenomic epidemiology of MRSA infection and colonization isolates among military trainees with skin and soft tissue infection.<h4>Purpose</h4>Individuals with methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI) can be simultaneously colonized with MRSA on multiple body sites. Using whole genome sequencing (WGS), the intrahost relatedness of MRSA colonization and infection isolates was investigated.<h4>Methods</h4>In the context of a prospective case-control study of SSTI, we analyzed colonization and infection isolates from US Army Infantry trainees with purulent infection due to MRSA. At the time of clinical presentation for SSTI, culture swabs were obtained from the infection site, as well as from the patient's nasal, oral, inguinal, and perianal regions. S. aureus culture and susceptibility was performed by standard methods. DNA from MRSA isolates was extracted and librarie2019-01-01T00:00:00Z2019 Oct2026-04-08T19:52:23.15Z2025-04-05T22:07:11.642ZS-EPMC119157513079662810.1007/s15010-019-01282-w