biostudies-literatureSun YNIHNIH HHSKavli Institute for Brain and Mind Postdoctoral Scholar awardjkaf004https://www.ebi.ac.uk/biostudies/studies/S-EPMC11917482biostudies-literatureUnknown15(3)The conserved MAP3K DLKs are widely known for their functions in synapse formation, axonal regeneration and degeneration, and neuronal survival, notably under traumatic injury and chronic disease conditions. In contrast, their roles in other neuronal compartments are much less explored. Through an unbiased forward genetic screening in C. elegans for altered patterns of GFP-tagged DLK-1 expressed from the endogenous locus, we have recently uncovered a mechanism by which the abundance of DLK-1 is tightly regulated by intraflagellar transport in ciliated sensory neurons. Here, we report additional mutants identified from the genetic screen. Most mutants exhibit increased accumulation of GFP::DLK-1 in sensory endings, and the levels of misaccumulated GFP::DLK-1 are exacerbated by loss of functG3 (Bethesda, Md.)Multiple regulators constrain the abundance of Caenorhabditis elegans DLK-1 in ciliated sensory neurons.PMC11917482NS R37 035546NS R35 127314P40 OD010440Debnath AJin YSun YWang ZZhou JXie BfalseMultiple regulators constrain the abundance of Caenorhabditis elegans DLK-1 in ciliated sensory neurons.The conserved MAP3K DLKs are widely known for their functions in synapse formation, axonal regeneration and degeneration, and neuronal survival, notably under traumatic injury and chronic disease conditions. In contrast, their roles in other neuronal compartments are much less explored. Through an unbiased forward genetic screening in C. elegans for altered patterns of GFP-tagged DLK-1 expressed from the endogenous locus, we have recently uncovered a mechanism by which the abundance of DLK-1 is tightly regulated by intraflagellar transport in ciliated sensory neurons. Here, we report additional mutants identified from the genetic screen. Most mutants exhibit increased accumulation of GFP::DLK-1 in sensory endings, and the levels of misaccumulated GFP::DLK-1 are exacerbated by loss of funct2025-01-01T00:00:00Z2025 Mar2026-07-15T11:12:09.98Z2025-04-05T22:07:23.258ZS-EPMC119174823985427310.1093/g3journal/jkaf004