biostudies-literatureUnknown16Hu X<h4>Introduction</h4>The oil content of tobacco leaves is intimately associated with their aromatic characteristics. This study aims to explore the microstructure and distinctive flavor substances of Yunyan 87 high-oil-content tobacco leaves.<h4>Methods</h4>The microstructure and characteristic flavor substances of Yunyan 87 tobacco leaves with different oil contents were analyzed using scanning electron microscope (SEM) and comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOF MS).<h4>Results</h4>The results indicate that the surface of high-oil tobacco leaves was characterized by a high density of glandular hairs, primarily composed of short-stalked glandular hairs featuring enlarged glandular heads. A total of 1551 flavor substances weFrontiers in plant science1537924https://www.ebi.ac.uk/biostudies/studies/S-EPMC11920166biostudies-literatureMicrostructure observation and flavor substances excavation of Yunyan 87 tobacco leaves with different oil contents.PMC11920166Deng DXie YPan SZhang YHu XLiao RXu WYang SWu YfalseMicrostructure observation and flavor substances excavation of Yunyan 87 tobacco leaves with different oil contents.<h4>Introduction</h4>The oil content of tobacco leaves is intimately associated with their aromatic characteristics. This study aims to explore the microstructure and distinctive flavor substances of Yunyan 87 high-oil-content tobacco leaves.<h4>Methods</h4>The microstructure and characteristic flavor substances of Yunyan 87 tobacco leaves with different oil contents were analyzed using scanning electron microscope (SEM) and comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOF MS).<h4>Results</h4>The results indicate that the surface of high-oil tobacco leaves was characterized by a high density of glandular hairs, primarily composed of short-stalked glandular hairs featuring enlarged glandular heads. A total of 1551 flavor substances we2025-01-01T00:00:00Z20252025-04-04T00:07:05.939Z2025-04-04T00:07:05.939ZS-EPMC119201664011035810.3389/fpls.2025.1537924