{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lau HL"],"funding":["Research Grants Council of the Hong Kong SAR, China Projects","NSFC Excellent Young Scientists Fund Project","State Key Laboratory of Marine Pollution Seed Collaborative Research Fund","City University of Hong Kong projects","Croucher Foundation"],"pagination":["e2401097"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11926469"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(3)"],"pubmed_abstract":["RNA G-quadruplex structures (rG4s) play important roles in the regulation of biological processes. So far, all the l-RNA aptamers developed to target rG4 of interest contain G4 motif itself, raising the question of whether non-G4-containing l-RNA aptamer can be developed to target rG4. Furthermore, it is unclear whether an l-Aptamer-based tool can be generated for G4 detection in vitro and imaging in cells. Herein, a new strategy is designed using a low GC content template library to develop a novel non-G4-containing l-RNA aptamer with strong binding affinity and improved binding specificity to rG4 of interest. The first non-G4-containing l-Aptamer, l-Apt.1-1, is identified with nanomolar binding affinity to amyloid precursor protein (APP) D-rG4. l-Apt.1-1 is applied to control APP gene ex"],"journal":["Small methods"],"pubmed_title":["Specific Targeting and Imaging of RNA G-Quadruplex (rG4) Structure Using Non-G4-Containing l-RNA Aptamer and Fluorogenic l-Aptamer."],"pmcid":["PMC11926469"],"funding_grant_id":["CityU11100123","CityU11100222","SCRF/0037","32222089","CityU11100421","6000827","RFS2425-1S02","SCRF/0070","SCRF/0040","9678302","9509003","7030001"],"pubmed_authors":["Kwok CK","Feng H","Lau HL","Zhao H"],"additional_accession":[]},"is_claimable":false,"name":"Specific Targeting and Imaging of RNA G-Quadruplex (rG4) Structure Using Non-G4-Containing l-RNA Aptamer and Fluorogenic l-Aptamer.","description":"RNA G-quadruplex structures (rG4s) play important roles in the regulation of biological processes. So far, all the l-RNA aptamers developed to target rG4 of interest contain G4 motif itself, raising the question of whether non-G4-containing l-RNA aptamer can be developed to target rG4. Furthermore, it is unclear whether an l-Aptamer-based tool can be generated for G4 detection in vitro and imaging in cells. Herein, a new strategy is designed using a low GC content template library to develop a novel non-G4-containing l-RNA aptamer with strong binding affinity and improved binding specificity to rG4 of interest. The first non-G4-containing l-Aptamer, l-Apt.1-1, is identified with nanomolar binding affinity to amyloid precursor protein (APP) D-rG4. l-Apt.1-1 is applied to control APP gene ex","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-04-20T00:14:20.412Z","creation":"2025-04-20T00:14:20.412Z"},"accession":"S-EPMC11926469","cross_references":{"pubmed":["39224911"],"doi":["10.1002/smtd.202401097"]}}