{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kelebeev J"],"funding":["York University","Heart and Stroke Foundation of Canada","Institute of Musculoskeletal Health and Arthritis","CIHR"],"pagination":["jcs263527"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11928053"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["138(4)"],"pubmed_abstract":["Characterization of protein-protein interactions (PPIs) is a fundamental goal in the post-genomic era. Here, we document a generally applicable approach to identify cellular protein interactomes using a combination of nanobody-based affinity purification (AP) coupled with liquid chromatography and tandem mass spectrometry (LC-MS/MS). The Hippo signaling regulator TAZ (also known as WWTR1) functions as a transcriptional co-repressor or activator depending on its PPI network; we therefore undertook an unbiased proteomic screen to identify TAZ PPIs in striated muscle cells. A GFP nanotrap-based AP approach coupled with protein identification through LC-MS/MS was used to document a comprehensive list of known and novel TAZ interactome components. Informatic analysis of the interactome document"],"journal":["Journal of cell science"],"pubmed_title":["TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry."],"pmcid":["PMC11928053"],"funding_grant_id":["PJT-159644","PJT 159644"],"pubmed_authors":["MacKeracher A","Kelebeev J","McDermott JC","Miyake T"],"additional_accession":[]},"is_claimable":false,"name":"TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry.","description":"Characterization of protein-protein interactions (PPIs) is a fundamental goal in the post-genomic era. Here, we document a generally applicable approach to identify cellular protein interactomes using a combination of nanobody-based affinity purification (AP) coupled with liquid chromatography and tandem mass spectrometry (LC-MS/MS). The Hippo signaling regulator TAZ (also known as WWTR1) functions as a transcriptional co-repressor or activator depending on its PPI network; we therefore undertook an unbiased proteomic screen to identify TAZ PPIs in striated muscle cells. A GFP nanotrap-based AP approach coupled with protein identification through LC-MS/MS was used to document a comprehensive list of known and novel TAZ interactome components. Informatic analysis of the interactome document","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-06-01T14:42:25.364Z","creation":"2026-04-08T13:21:55.592Z"},"accession":"S-EPMC11928053","cross_references":{"pubmed":["39898439"],"doi":["10.1242/jcs.263527"]}}