{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang L"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Guangdong Province"],"pagination":["103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11929334"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(1)"],"pubmed_abstract":["<h4>Background</h4>Perineural invasion (PNI) is a hallmark feature of pancreatic ductal adenocarcinoma (PDAC), which occurs at a high incidence and significantly contributes to PDAC lethality and poor survival. Despite its prevalence and association with poor prognosis, the molecular mechanisms underlying PNI in PDAC remain unclear.<h4>Methods</h4>We investigated clinical samples from two cohorts by UPLC/MS-MS to profiled significantly altered chemical RNA modifications in PDAC tissues with PNI lesions. Dorsal root ganglion coculture systems and sciatic nerve injection models validated PNI ability. We combined RNA-seq, acRIP-seq and ac4C-seq with CRISPR-based techniques to explore the regulatory mechanism of ac4C modification on the integrin beta 5 (ITGB5) transcript.<h4>Result</h4>We repo"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["N&lt;sup&gt;4&lt;/sup&gt;-acetylcytidine modification of ITGB5 mRNA mediated by NAT10 promotes perineural invasion in pancreatic ductal adenocarcinoma."],"pmcid":["PMC11929334"],"funding_grant_id":["82073149","2022A1515220219","82472893"],"pubmed_authors":["Liu J","Li J","Zhou Q","Lu Y","Guo X","Fu Z","Huang L","Zhou Y","He R","Chen R","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"N&lt;sup&gt;4&lt;/sup&gt;-acetylcytidine modification of ITGB5 mRNA mediated by NAT10 promotes perineural invasion in pancreatic ductal adenocarcinoma.","description":"<h4>Background</h4>Perineural invasion (PNI) is a hallmark feature of pancreatic ductal adenocarcinoma (PDAC), which occurs at a high incidence and significantly contributes to PDAC lethality and poor survival. Despite its prevalence and association with poor prognosis, the molecular mechanisms underlying PNI in PDAC remain unclear.<h4>Methods</h4>We investigated clinical samples from two cohorts by UPLC/MS-MS to profiled significantly altered chemical RNA modifications in PDAC tissues with PNI lesions. Dorsal root ganglion coculture systems and sciatic nerve injection models validated PNI ability. We combined RNA-seq, acRIP-seq and ac4C-seq with CRISPR-based techniques to explore the regulatory mechanism of ac4C modification on the integrin beta 5 (ITGB5) transcript.<h4>Result</h4>We repo","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-06-01T22:57:17.246Z","creation":"2025-07-04T03:06:02.629Z"},"accession":"S-EPMC11929334","cross_references":{"pubmed":["40119353"],"doi":["10.1186/s13046-025-03362-2"]}}