{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["62"],"submitter":["Wu SR"],"pubmed_abstract":["Diffuse midline gliomas (DMGs) are lethal brain tumors that arise in children and young adults, resulting in a median survival of less than two years. Genetically engineered mouse models (GEMMs) are critical to studying tumorigenesis and tumor-immune interactions, which may inform new treatment approaches. However, current midline glioma GEMM approaches are limited in their ability to multiplex perturbations and/or target specific cell lineages in the brain for genetic manipulation. Here, we combined the RCAS/tv-a avian retrovirus system and CRISPR/Cas9 genetic engineering to drive midline glioma formation in mice. CRISPR/Cas9-based disruption of Trp53, a tumor suppressor that is frequently disrupted in midline gliomas, along with the oncogene PDGF-B resulted in high grade tumor formation "],"journal":["Neoplasia (New York, N.Y.)"],"pagination":["101139"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11930108"],"repository":["biostudies-literature"],"pubmed_title":["Combining the RCAS/tv-a retrovirus and CRISPR/Cas9 gene editing systems to generate primary mouse models of diffuse midline glioma."],"pmcid":["PMC11930108"],"pubmed_authors":["Guerra Garcia ME","Valentine V","Groth AJ","Jacob S","Williams NT","Reitman ZJ","Chen R","Wu SR","Sharpe J","Tolliver J"],"additional_accession":[]},"is_claimable":false,"name":"Combining the RCAS/tv-a retrovirus and CRISPR/Cas9 gene editing systems to generate primary mouse models of diffuse midline glioma.","description":"Diffuse midline gliomas (DMGs) are lethal brain tumors that arise in children and young adults, resulting in a median survival of less than two years. Genetically engineered mouse models (GEMMs) are critical to studying tumorigenesis and tumor-immune interactions, which may inform new treatment approaches. However, current midline glioma GEMM approaches are limited in their ability to multiplex perturbations and/or target specific cell lineages in the brain for genetic manipulation. Here, we combined the RCAS/tv-a avian retrovirus system and CRISPR/Cas9 genetic engineering to drive midline glioma formation in mice. CRISPR/Cas9-based disruption of Trp53, a tumor suppressor that is frequently disrupted in midline gliomas, along with the oncogene PDGF-B resulted in high grade tumor formation ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-02T03:04:23.746Z","creation":"2025-07-02T03:04:23.746Z"},"accession":"S-EPMC11930108","cross_references":{"pubmed":["40056601"],"doi":["10.1016/j.neo.2025.101139"]}}