biostudies-literatureUnknown7(4)Wang C<h4>Background & aims</h4>Chronic HBV infection usually causes cirrhosis and hepatocellular carcinoma. Comparative investigations of acute and chronic HBV cases would help determine the immune responses crucial for viral clearance.<h4>Methods</h4>A fast-cleared HBV mouse model was established in Alb-Cre mice via hydrodynamic injection of HBV plasmid, while persistent HBV model mice were generated via recombinant covalently closed circular DNA-adeno-associated virus 8 infection. The single-cell transcriptomes of CD45<sup>+</sup> intrahepatic non-parenchymal cells from these mice were conducted. Multiplexed immunohistochemistry and flow cytometry were used to confirm the findings from single-cell transcriptomes. Transwell, coculture, and adoptive transfer experiments were performed to study JHEP reports : innovation in hepatology101323https://www.ebi.ac.uk/biostudies/studies/S-EPMC11937660biostudies-literaturePotential role of liver-resident CD3&lt;sup&gt;+&lt;/sup&gt; macrophages in HBV clearance in a mouse hepatitis B model.PMC11937660Li YWu MFang ZShi BWang CZhang XZai WZhao KZhou XYuan ZKozlowski MfalsePotential role of liver-resident CD3&lt;sup&gt;+&lt;/sup&gt; macrophages in HBV clearance in a mouse hepatitis B model.<h4>Background & aims</h4>Chronic HBV infection usually causes cirrhosis and hepatocellular carcinoma. Comparative investigations of acute and chronic HBV cases would help determine the immune responses crucial for viral clearance.<h4>Methods</h4>A fast-cleared HBV mouse model was established in Alb-Cre mice via hydrodynamic injection of HBV plasmid, while persistent HBV model mice were generated via recombinant covalently closed circular DNA-adeno-associated virus 8 infection. The single-cell transcriptomes of CD45<sup>+</sup> intrahepatic non-parenchymal cells from these mice were conducted. Multiplexed immunohistochemistry and flow cytometry were used to confirm the findings from single-cell transcriptomes. Transwell, coculture, and adoptive transfer experiments were performed to study 2025-01-01T00:00:00Z2025 Apr2025-06-25T03:04:30.102Z2025-06-25T03:04:30.102ZS-EPMC119376604014394810.1016/j.jhepr.2024.101323