biostudies-literatureZebene EDMinistero della SaluteInternational Atomic Energy Agency1010https://www.ebi.ac.uk/biostudies/studies/S-EPMC11940482biostudies-literatureUnknown17(6)<h4>Background</h4>Ionizing radiation (IR) exposure poses a significant health risk due to its widespread use in medical diagnostics and therapeutic applications, necessitating rapid and effective biomarkers for assessment.<h4>Objective</h4>The aim of this study is to identify the serum proteomic signature of IR exposure in patients undergoing radiotherapy (RT).<h4>Methods</h4>Blood samples were obtained from eighteen patients with head and neck cancer (HNC) and five patients with rectal cancer before and immediately after they underwent curative intensity-modulated radiotherapy (IMRT). The comprehensive serum proteome was analyzed in individual samples using nanoHPLC-MS/MS.<h4>Results</h4>Forty radiation-modulated proteins (RMPs), 24 upregulated and 16 downregulated, with a fold change ≥1CancersSerum-Based Proteomic Approach to Identify Clinical Biomarkers of Radiation Exposure.PMC11940482RC2024 L2/2ETH/6023Di Gennaro EWoldemichael GBBudillon AZebene EDMedhin HTLombardi RPucci BSeife EfalseSerum-Based Proteomic Approach to Identify Clinical Biomarkers of Radiation Exposure.<h4>Background</h4>Ionizing radiation (IR) exposure poses a significant health risk due to its widespread use in medical diagnostics and therapeutic applications, necessitating rapid and effective biomarkers for assessment.<h4>Objective</h4>The aim of this study is to identify the serum proteomic signature of IR exposure in patients undergoing radiotherapy (RT).<h4>Methods</h4>Blood samples were obtained from eighteen patients with head and neck cancer (HNC) and five patients with rectal cancer before and immediately after they underwent curative intensity-modulated radiotherapy (IMRT). The comprehensive serum proteome was analyzed in individual samples using nanoHPLC-MS/MS.<h4>Results</h4>Forty radiation-modulated proteins (RMPs), 24 upregulated and 16 downregulated, with a fold change ≥12025-01-01T00:00:00Z2025 Mar2026-04-18T03:15:18.591Z2025-07-10T03:08:33.424ZS-EPMC119404824014934410.3390/cancers17061010