biostudies-literatureLe Minh GNCATS NIH HHSNCI NIH HHSNIH HHSNIGMS NIH HHS398https://www.ebi.ac.uk/biostudies/studies/S-EPMC11941746biostudies-literatureUnknown14(6)The growth of breast tumors is driven and controlled by a subpopulation of cancer cells resembling adult stem cells, which are called cancer stem-like cells (CSCs). In breast cancer, the function and maintenance of CSCs are influenced by protein O-GlcNAcylation and the enzyme responsible for this post-translational modification, O-GlcNAc transferase (OGT). However, the mechanism of CSCs regulation by OGT and O-GlcNAc cycling in breast cancer is still unclear. Analysis of the proteome and O-GlcNAcome, revealed GATAD2B, a component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, as a substrate regulated by OGT. Reducing GATAD2B genetically impairs mammosphere formation, decreases expression of self-renewal factors and CSCs population. O-GlcNAcylation of GATAD2B at the C-terminusCellsGATAD2B O-GlcNAcylation Regulates Breast Cancer Stem-like Potential and Drug Resistance.PMC11941746P30 CA138313UL1 TR001450S10 OD0286921U01CA244303-24A5P20 GM103542U01 CA2443031P30CA138313-24A51UL1TR001450-24A31T32CA193201-24A2S10 OD0251261P20GM103542-24A3T32 CA193201Lee MHComte-Walters SEsquea EMSharp RJAhmed NNLe Minh GDhameliya TTAgana BReginato MJBethard JRMerzy JYoung RGBall LEfalseGATAD2B O-GlcNAcylation Regulates Breast Cancer Stem-like Potential and Drug Resistance.The growth of breast tumors is driven and controlled by a subpopulation of cancer cells resembling adult stem cells, which are called cancer stem-like cells (CSCs). In breast cancer, the function and maintenance of CSCs are influenced by protein O-GlcNAcylation and the enzyme responsible for this post-translational modification, O-GlcNAc transferase (OGT). However, the mechanism of CSCs regulation by OGT and O-GlcNAc cycling in breast cancer is still unclear. Analysis of the proteome and O-GlcNAcome, revealed GATAD2B, a component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, as a substrate regulated by OGT. Reducing GATAD2B genetically impairs mammosphere formation, decreases expression of self-renewal factors and CSCs population. O-GlcNAcylation of GATAD2B at the C-terminus2025-01-01T00:00:00Z2025 Mar2026-04-15T03:28:28.707Z2026-04-15T03:15:25.353ZS-EPMC119417464013664710.3390/cells14060398