{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Imeidopf G"],"funding":["NIBIB NIH HHS"],"pagination":["2752"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11942841"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(6)"],"pubmed_abstract":["Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable and biocompatible alternative to synthetic grafts and autologous vessels. We refined decellularization protocols to achieve >95% DNA removal while preserving mechanical properties comparable to native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based and Trypsin/Tergitol-based treatments, achieved via clearing in bleach and Triton X-100 for 6 to 72 h. To assess the environmental influence on scaffold performance, leaves from multiple collection sites were processed using sodium dodecyl sulfate"],"journal":["International journal of molecular sciences"],"pubmed_title":["Optimization and Standardization of Plant-Derived Vascular Scaffolds."],"pmcid":["PMC11942841"],"funding_grant_id":["R15EB033168"],"pubmed_authors":["Khaimov D","Merna N","John S","Imeidopf G"],"additional_accession":[]},"is_claimable":false,"name":"Optimization and Standardization of Plant-Derived Vascular Scaffolds.","description":"Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable and biocompatible alternative to synthetic grafts and autologous vessels. We refined decellularization protocols to achieve >95% DNA removal while preserving mechanical properties comparable to native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based and Trypsin/Tergitol-based treatments, achieved via clearing in bleach and Triton X-100 for 6 to 72 h. To assess the environmental influence on scaffold performance, leaves from multiple collection sites were processed using sodium dodecyl sulfate","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-07-05T03:04:18.491Z","creation":"2025-07-05T03:04:18.491Z"},"accession":"S-EPMC11942841","cross_references":{"pubmed":["40141394"],"doi":["10.3390/ijms26062752"]}}