biostudies-literatureImeidopf GNIBIB NIH HHS2752https://www.ebi.ac.uk/biostudies/studies/S-EPMC11942841biostudies-literatureUnknown26(6)Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable and biocompatible alternative to synthetic grafts and autologous vessels. We refined decellularization protocols to achieve >95% DNA removal while preserving mechanical properties comparable to native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based and Trypsin/Tergitol-based treatments, achieved via clearing in bleach and Triton X-100 for 6 to 72 h. To assess the environmental influence on scaffold performance, leaves from multiple collection sites were processed using sodium dodecyl sulfateInternational journal of molecular sciencesOptimization and Standardization of Plant-Derived Vascular Scaffolds.PMC11942841R15EB033168Khaimov DMerna NJohn SImeidopf GfalseOptimization and Standardization of Plant-Derived Vascular Scaffolds.Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable and biocompatible alternative to synthetic grafts and autologous vessels. We refined decellularization protocols to achieve >95% DNA removal while preserving mechanical properties comparable to native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based and Trypsin/Tergitol-based treatments, achieved via clearing in bleach and Triton X-100 for 6 to 72 h. To assess the environmental influence on scaffold performance, leaves from multiple collection sites were processed using sodium dodecyl sulfate2025-01-01T00:00:00Z2025 Mar2025-07-05T03:04:18.491Z2025-07-05T03:04:18.491ZS-EPMC119428414014139410.3390/ijms26062752