biostudies-literatureSong XWancang SunGang YangJunyan WuWangtian Wang2737https://www.ebi.ac.uk/biostudies/studies/S-EPMC11942965biostudies-literatureUnknown26(6)Glyoxalase I (GLYI) is a key enzyme that detoxifies methylglyoxal, a toxic byproduct of glycolysis, and is essential for plant pollination. However, the genome-wide identification and functional analysis of <i>GLYI</i> in <i>Brassica rapa</i> L. (<i>B. rapa</i>) remain limited. This study identified 17 <i>BrGLYI</i> genes (<i>BrGLYI1</i>-<i>BrGLYI17</i>) from the <i>B. rapa</i> genome. The self-compatible line 039-1 and the self-incompatible line GAU-28-5 were used as experimental materials, and Real-Time Quantitative Reverse Transcription PCR (RT-qPCR) was performed to examine the effect of <i>BrGLYI</i> genes on self-compatibility in winter <i>B. rapa</i>. Preliminary results showed that <i>BrGLYI13</i> exhibited significant tissue specificity, with higher expression in the flowers of 03International journal of molecular sciencesCloning and Functional Analysis of Glyoxalase I Gene <i>BrGLYI 13</i> in <i>Brassica rapa</i> L.PMC11942965CARS-12-09GAU-KYQD-2022-09Grant No. 2024YFD2300204-0822ZD6NA009Zhang FWu JYang GLi YMa LLiu LTao XPu YWang WSun WSong XFan TfalseCloning and Functional Analysis of Glyoxalase I Gene <i>BrGLYI 13</i> in <i>Brassica rapa</i> L.Glyoxalase I (GLYI) is a key enzyme that detoxifies methylglyoxal, a toxic byproduct of glycolysis, and is essential for plant pollination. However, the genome-wide identification and functional analysis of <i>GLYI</i> in <i>Brassica rapa</i> L. (<i>B. rapa</i>) remain limited. This study identified 17 <i>BrGLYI</i> genes (<i>BrGLYI1</i>-<i>BrGLYI17</i>) from the <i>B. rapa</i> genome. The self-compatible line 039-1 and the self-incompatible line GAU-28-5 were used as experimental materials, and Real-Time Quantitative Reverse Transcription PCR (RT-qPCR) was performed to examine the effect of <i>BrGLYI</i> genes on self-compatibility in winter <i>B. rapa</i>. Preliminary results showed that <i>BrGLYI13</i> exhibited significant tissue specificity, with higher expression in the flowers of 032025-01-01T00:00:00Z2025 Mar2025-07-02T03:04:50.425Z2025-07-02T03:04:50.425ZS-EPMC119429654014137910.3390/ijms26062737