biostudies-literatureYao XZhengfu ZhouYuhua ZhanYongliang YanMin Lin552https://www.ebi.ac.uk/biostudies/studies/S-EPMC11944486biostudies-literatureUnknown13(3)Biotechnological research and application of microbial enzyme production have consistently been focal points for scientific inquiry and industrial advancement. In this study, <i>Bacillus subtilis</i> Y4X3 was isolated from saline-alkaline soil in Xinjiang, China. Extracellular enzyme production analysis revealed that <i>B. subtilis</i> Y4X3 can secrete various enzymes, including cellulase, xylanase, protease, and amylase. Sequencing and assembly of the complete genome of this strain revealed a genome size of 4,215,636 bp with 43.51% C + G content, including 4438 coding genes. Genome annotation was performed with databases to predict gene functions in <i>B. subtilis</i> Y4X3, and a variety of genes related to carbohydrate metabolism were identified. A cellulase-encoding gene was subsequentlMicroorganismsGenomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing Strain <i>Bacillus subtilis</i> Y4X3 Isolated from Saline-Alkaline Soil in Xinjiang, China.PMC119444862023YFF10003002022xjkk020602323700912023YFF100030432270067CAAS-ZDRW202305Su BJiang SYao XYan YWang JLin MZhan YZhou ZfalseGenomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing Strain <i>Bacillus subtilis</i> Y4X3 Isolated from Saline-Alkaline Soil in Xinjiang, China.Biotechnological research and application of microbial enzyme production have consistently been focal points for scientific inquiry and industrial advancement. In this study, <i>Bacillus subtilis</i> Y4X3 was isolated from saline-alkaline soil in Xinjiang, China. Extracellular enzyme production analysis revealed that <i>B. subtilis</i> Y4X3 can secrete various enzymes, including cellulase, xylanase, protease, and amylase. Sequencing and assembly of the complete genome of this strain revealed a genome size of 4,215,636 bp with 43.51% C + G content, including 4438 coding genes. Genome annotation was performed with databases to predict gene functions in <i>B. subtilis</i> Y4X3, and a variety of genes related to carbohydrate metabolism were identified. A cellulase-encoding gene was subsequentl2025-01-01T00:00:00Z2025 Feb2025-06-25T03:04:32.465Z2025-06-25T03:04:32.465ZS-EPMC119444864014244510.3390/microorganisms13030552