biostudies-literatureHan YNNatural Science Foundation of Shandong Province, ChinaScience and Technology Support Plan for Youth Innovation in Universities of Shandong ProvinceNational Natural Science Foundation of ChinaShandong Laboratory ProgramFundamental Research Projects of Science & Technology Innovation and development Plan in Yantai CityTaishan Scholars ProgramKey R&D Program of Shandong Province, ChinaYoung Elite Scientists Sponsorship Program by Chinese Chemical Society1221https://www.ebi.ac.uk/biostudies/studies/S-EPMC11945828biostudies-literatureUnknown30(6)<i>β</i>-galactosidase (<i>β</i>-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development of a fluorescent sensor is of considerable importance for precisely detecting the activity and spatial distribution of <i>β</i>-Gal. In this study, we developed two excited-state-altering responsive fluorescent sensors (<b>TF1</b> and <b>TF2</b>) for ratiometric detection of <i>β</i>-Gal. Two TCF dyes, composed of tricyanofuran (TCF) and naphthol units, feature electron "pull-push" systems and are quenched fluorescence by <i>β</i>-Gal. Upon <i>β</i>-Gal hydrolysis, a significant ratiometric shift in absorption from ca. 475 nm to 630 nm is observed, accompanied by the emergence of a fluorescence signal at ca. 660 nm. ThMolecules (Basel, Switzerland)Excited-State-Altering Ratiometric Fluorescent Probes for the Response of <i>β</i>-Galactosidase in Senescent Cells.PMC11945828tsqn202312305221070292024KJG029ZR2023QB2482024CXPT0282237713522305178NonSYS2022052023JCYJ059Sun LLLi CZhang ZHan YNXie JDong LLi WJRen SHan HHfalseExcited-State-Altering Ratiometric Fluorescent Probes for the Response of <i>β</i>-Galactosidase in Senescent Cells.<i>β</i>-galactosidase (<i>β</i>-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development of a fluorescent sensor is of considerable importance for precisely detecting the activity and spatial distribution of <i>β</i>-Gal. In this study, we developed two excited-state-altering responsive fluorescent sensors (<b>TF1</b> and <b>TF2</b>) for ratiometric detection of <i>β</i>-Gal. Two TCF dyes, composed of tricyanofuran (TCF) and naphthol units, feature electron "pull-push" systems and are quenched fluorescence by <i>β</i>-Gal. Upon <i>β</i>-Gal hydrolysis, a significant ratiometric shift in absorption from ca. 475 nm to 630 nm is observed, accompanied by the emergence of a fluorescence signal at ca. 660 nm. Th2025-01-01T00:00:00Z2025 Mar2025-07-06T03:04:34.507Z2025-07-06T03:04:34.507ZS-EPMC119458284014199810.3390/molecules30061221