biostudies-literatureUnknown105(6)Pipponzi S<h4>Background</h4>Vitamin B₁₂ (cobalamin) can be produced de novo only by certain bacteria and archaea. It plays a crucial role in the health of animals and humans, which obtain it only through diet, mainly from animal products. This study aimed to identify endophytic bacterial strains capable of synthesizing vitamin B₁₂ and enriching edible plants with it as a potential solution for vitamin B₁₂ deficiency in vegetarians, vegans, and people with poor diets.<h4>Results</h4>An in silico genome analysis was performed on 66 bacterial genomes, including the reference strain Pseudomonas denitrificans ATCC 13867, a known vitamin B₁₂ producer. The genomes were analyzed using the Rapid Annotations using Subsystems Technology (RAST) server and the MetaCycJournal of the science of food and agriculture3343-3354https://www.ebi.ac.uk/biostudies/studies/S-EPMC11949862biostudies-literatureLettuce fortification through vitamin B&lt;sub&gt;12&lt;/sub&gt;-producing bacteria - proof of concept study.PMC11949862Antonielli LSessitsch ACompant SPipponzi SStefani EPrimisser SKostic TfalseLettuce fortification through vitamin B&lt;sub&gt;12&lt;/sub&gt;-producing bacteria - proof of concept study.<h4>Background</h4>Vitamin B₁₂ (cobalamin) can be produced de novo only by certain bacteria and archaea. It plays a crucial role in the health of animals and humans, which obtain it only through diet, mainly from animal products. This study aimed to identify endophytic bacterial strains capable of synthesizing vitamin B₁₂ and enriching edible plants with it as a potential solution for vitamin B₁₂ deficiency in vegetarians, vegans, and people with poor diets.<h4>Results</h4>An in silico genome analysis was performed on 66 bacterial genomes, including the reference strain Pseudomonas denitrificans ATCC 13867, a known vitamin B₁₂ producer. The genomes were analyzed using the Rapid Annotations using Subsystems Technology (RAST) server and the MetaCyc2025-01-01T00:00:00Z2025 Apr2026-06-01T05:12:38.049Z2025-06-25T03:04:34.309ZS-EPMC119498623983155610.1002/jsfa.14095