biostudies-literatureWang ZNational Natural Science Foundation of ChinaNational Key Research and Development Program of China10917https://www.ebi.ac.uk/biostudies/studies/S-EPMC11954891biostudies-literatureUnknown15(1)Primary human aortic valvular interstitial cells (pHAVICs) play crucial roles in maintaining the mechanical structure and microenvironmental homeostasis of aortic valves. Pathologic processes such as inflammation, senescence, apoptosis, and metabolic disorders of valvular interstitial cells often lead to calcified aortic valve disease (CAVD). However, the lack of clinically relevant cellular models has impeded our understanding of CAVD. Here, we immortalized primary HAVICs with SV40 LTA. The iHAVICs (immortalized human aortic valvular interstitial cells) were maintained in a nonsenescent state and still had the potential to be induced into a senescent phenotype. In calcification induction experiments, iHAVICs can be induced to transform into osteogenic phenotypes via different stimuli via Scientific reportsEstablishment and characterization of a novel immortalized human aortic valve interstitial cell line.PMC11954891824704232021YFA1101900Rao ZDong NWang YWang ZfalseEstablishment and characterization of a novel immortalized human aortic valve interstitial cell line.Primary human aortic valvular interstitial cells (pHAVICs) play crucial roles in maintaining the mechanical structure and microenvironmental homeostasis of aortic valves. Pathologic processes such as inflammation, senescence, apoptosis, and metabolic disorders of valvular interstitial cells often lead to calcified aortic valve disease (CAVD). However, the lack of clinically relevant cellular models has impeded our understanding of CAVD. Here, we immortalized primary HAVICs with SV40 LTA. The iHAVICs (immortalized human aortic valvular interstitial cells) were maintained in a nonsenescent state and still had the potential to be induced into a senescent phenotype. In calcification induction experiments, iHAVICs can be induced to transform into osteogenic phenotypes via different stimuli via 2025-01-01T00:00:00Z2025 Mar2025-07-10T03:08:22.224Z2025-07-10T03:08:22.224ZS-EPMC119548914015792710.1038/s41598-025-85909-z