biostudies-literatureGuangbin PNational Natural Science Foundation of China: Study on the Protective Mechanism of Moxibustion on Intestinal Mucosal Barrier in Ulcerative Colitis based on GABAergic SystemState Administration of Traditional Chinese Medicine High-level Key Discipline Construction ProjectShanghai Sailing Program: to Study the Protective Effect of Moxibustion on Intestinal Mucosal Barrier in Crohn's Disease Based on Histone H3 Acetylation ModificationNational Natural Science Foundation of China: Study for the Mechanism of Moxibustion in Ulcerative Colitis based on the α7 Nicotinic Acetylcholine Receptor Mediated Cholinergic Anti-inflammatory PathwayNational Natural Science Foundation of China: Moxibustion Regulates P300-mediated Histone H3K27 Acetylation Modification in the Treatment of Crohn's DiseaseNational Natural Science Foundation of China: Study of the Central Nervous System Regulatory Mechanism of Moxibustion Repair of Ulcerative Colitis Gut Vascular Barrier Based on Ubiquitin Specific Peptidase 14 Deubiquitination368-375https://www.ebi.ac.uk/biostudies/studies/S-EPMC11955752biostudies-literatureUnknown45(2)<h4>Objective</h4>To investigate the effect and mechanism of mild moxibustion on the non-neuronal cholinergic system (NNCS) in rats with ulcerative colitis (UC).<h4>Methods</h4>UC rat model was established by administering 4% dextran sulfate sodium. After 7 d, mild moxibustion, α7 nicotinic acetylcholine receptors (α7nAchRs) antagonist (α-bungarotoxin, α-BGT), vesicular acetylcholine transport inhibitor (vesamicol hydrochloride, VH) and organic cation transporters inhibitor (quinine, Qu) treatments were performed once daily for 7 d. Haematoxylin and eosin staining was used for morphological evaluation of colon tissues. Enzyme-linked immunosorbent assay (ELISA) was used to measure the protein expressions of interleukin-1β (IL-1β) and choline acetyltransferase (ChAT) in colon tissue. ReverseJournal of traditional Chinese medicine = Chung i tsa chih ying wen panRegulation of mild moxibustion on non-neuronal cholinergic system in ulcerative colitis rats.PMC119557528210501222YF1444100822746418220529382205262zyyzdxk-2023068Qin QIZhe MALinshan ZGuangbin PLu ZShiyu ZYaying LLuyi WUYan HHan LIHuangan WUfalseRegulation of mild moxibustion on non-neuronal cholinergic system in ulcerative colitis rats.<h4>Objective</h4>To investigate the effect and mechanism of mild moxibustion on the non-neuronal cholinergic system (NNCS) in rats with ulcerative colitis (UC).<h4>Methods</h4>UC rat model was established by administering 4% dextran sulfate sodium. After 7 d, mild moxibustion, α7 nicotinic acetylcholine receptors (α7nAchRs) antagonist (α-bungarotoxin, α-BGT), vesicular acetylcholine transport inhibitor (vesamicol hydrochloride, VH) and organic cation transporters inhibitor (quinine, Qu) treatments were performed once daily for 7 d. Haematoxylin and eosin staining was used for morphological evaluation of colon tissues. Enzyme-linked immunosorbent assay (ELISA) was used to measure the protein expressions of interleukin-1β (IL-1β) and choline acetyltransferase (ChAT) in colon tissue. Reverse2025-01-01T00:00:00Z2025 Apr2025-07-03T03:06:43.91Z2025-07-03T03:06:43.91ZS-EPMC119557524015112310.19852/j.cnki.jtcm.2025.02.013