biostudies-literatureXiying TScience Foundation of China Project: Involvement of Hypoxia Inducible Factor-1α Signal in Huangqi (Radix Astragali Mongolici)-Ezhu (Rhizoma Curcumae Phaeocaulis) Combination Induced Remolding of Tumor Hypoxic MicroenvironmentNational Natural Science Foundation of China Youth Found Project: the Mechanism of the Compatibility of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) on the Early Metastasis of Hepatocellular Carcinoma Mediated by Cancer Associated FibroblastsScience and Technology Development Project of Traditional Chinese Medicine in Jiangsu Province: Mechanism of Huangqi (Radix Astragali Mongolici)-Ezhu (Rhizoma Curcumae Phaeocaulis) Herb Pair on the Inhibition of Colon Cancer Metastasis Through the Wnt/β-catenin PathwayNational Natural Science Foundation of China Youth Found Project: Anti-Colorectal Cancer Metastasis Mechanism of Huangqi (Radix Astragali Mongolici)-Ezhu (Rhizoma Curcumae Phaeocaulis) According to the Hypoxia-Inducible Factor 2 Alpha/β-Catenin Cross-Talk Which Influence the Colon Tumor Stem Cells in Hypoxia Microenvironment385-398https://www.ebi.ac.uk/biostudies/studies/S-EPMC11955770biostudies-literatureUnknown45(2)<h4>Objective</h4>To evaluate the effects of Huangqi (<i>Radix Astragali Mongolici</i>)-Ezhu (<i>Rhizoma Curcumae Phaeocaulis</i>) (HQEZ) on colorectal cancer therapies and to elucidate the potential mechanisms of HQEZ, especially in combination with 5-Fluorouracil (5-FU).<h4>Methods</h4>The anti-tumor effects of HQEZ were evaluated in colorectal cancer models both <i>in vivo</i> and <i>in vitro</i>. The network pharmacological assay was used to investigate potential mechanisms of HQEZ. Potential target genes were selected by Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, protein-protein interaction network (PPI) and molecular docking. Within key targets, potential targets related to drug sensitivity, especially the sensitivity tJournal of traditional Chinese medicine = Chung i tsa chih ying wen panIntegrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models.PMC11955770821044088200396182074035YB201921Xiying TShuo ZYu ZRuxin GUJing TGang YRuiQian SDecai TfalseIntegrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models.<h4>Objective</h4>To evaluate the effects of Huangqi (<i>Radix Astragali Mongolici</i>)-Ezhu (<i>Rhizoma Curcumae Phaeocaulis</i>) (HQEZ) on colorectal cancer therapies and to elucidate the potential mechanisms of HQEZ, especially in combination with 5-Fluorouracil (5-FU).<h4>Methods</h4>The anti-tumor effects of HQEZ were evaluated in colorectal cancer models both <i>in vivo</i> and <i>in vitro</i>. The network pharmacological assay was used to investigate potential mechanisms of HQEZ. Potential target genes were selected by Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, protein-protein interaction network (PPI) and molecular docking. Within key targets, potential targets related to drug sensitivity, especially the sensitivity t2025-01-01T00:00:00Z2025 Apr2026-06-02T00:06:00.741Z2025-07-14T03:02:54.993ZS-EPMC119557704015112510.19852/j.cnki.jtcm.2025.02.004