biostudies-literatureUnknown17(12)Redolfi-Bristol DSelenium nanoparticles (SeNPs) exhibit significant potential in biomedical applications due to their antimicrobial, anticancer, and anti-inflammatory properties. In this study, we synthesized biocompatible SeNPs and employed confocal Raman microspectroscopy to map their distribution within human dermal fibroblast (HDF) cells. SeNPs possess a distinctive Raman band placed outside the cellular fingerprint region, which facilitates its detection and precise Raman imaging. Viability assays revealed that SeNPs exhibit cytotoxic effects only at the highest concentrations and for long exposure times while resulting in no harmful effects during all of the other treatments. For the first time, we achieved three-dimensional (3D) Raman mapping of SeNPs within cells, providing insights into their cellACS applied materials & interfaces18124-18133https://www.ebi.ac.uk/biostudies/studies/S-EPMC11956006biostudies-literatureMapping Selenium Nanoparticles Distribution Inside Cells through Confocal Raman Microspectroscopy.PMC11956006Yamamoto KMarin ERedolfi-Bristol DMazda OZhu WPezzotti GRiello PfalseMapping Selenium Nanoparticles Distribution Inside Cells through Confocal Raman Microspectroscopy.Selenium nanoparticles (SeNPs) exhibit significant potential in biomedical applications due to their antimicrobial, anticancer, and anti-inflammatory properties. In this study, we synthesized biocompatible SeNPs and employed confocal Raman microspectroscopy to map their distribution within human dermal fibroblast (HDF) cells. SeNPs possess a distinctive Raman band placed outside the cellular fingerprint region, which facilitates its detection and precise Raman imaging. Viability assays revealed that SeNPs exhibit cytotoxic effects only at the highest concentrations and for long exposure times while resulting in no harmful effects during all of the other treatments. For the first time, we achieved three-dimensional (3D) Raman mapping of SeNPs within cells, providing insights into their cell2025-01-01T00:00:00Z2025 Mar2025-07-02T03:04:26.014Z2025-07-02T03:04:26.014ZS-EPMC119560064009847510.1021/acsami.5c00380