{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Baronas D"],"funding":["NCI NIH HHS"],"pubmed_abstract":["Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput analytical methods and tools that efficiently probe the cellular phenotype and genotype. While recent advancements have enabled various single-cell -omics assays, their broader applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. Extending on our previous work (1) here we present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of high-throughput nucleic acid assays, including digital PCR, genome sequencing, single-cell RNA-sequencing (scRNA-Seq) and FACS-based isolation of individual transcriptomes based on nucleic acid marker"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.03.14.642805"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11957016"],"repository":["biostudies-literature"],"pubmed_title":["High-throughput single cell -omics using semi-permeable capsules."],"pmcid":["PMC11957016"],"funding_grant_id":["R33 CA278392"],"pubmed_authors":["Zvirblyte J","Mazutis L","Juzenas S","Goda K","Leonaviciene G","Sablauskas K","Baronas D","Norvaisis S","Mikulenaite V","Kaseta V","Griskevicius L"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput single cell -omics using semi-permeable capsules.","description":"Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput analytical methods and tools that efficiently probe the cellular phenotype and genotype. While recent advancements have enabled various single-cell -omics assays, their broader applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. Extending on our previous work (1) here we present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of high-throughput nucleic acid assays, including digital PCR, genome sequencing, single-cell RNA-sequencing (scRNA-Seq) and FACS-based isolation of individual transcriptomes based on nucleic acid marker","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-06-03T03:11:47.431Z","creation":"2025-07-09T03:05:00.593Z"},"accession":"S-EPMC11957016","cross_references":{"pubmed":["40166174"],"doi":["10.1101/2025.03.14.642805"]}}