biostudies-literatureUnknownBaronas DNCI NIH HHSBiological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput analytical methods and tools that efficiently probe the cellular phenotype and genotype. While recent advancements have enabled various single-cell -omics assays, their broader applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. Extending on our previous work (1) here we present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of high-throughput nucleic acid assays, including digital PCR, genome sequencing, single-cell RNA-sequencing (scRNA-Seq) and FACS-based isolation of individual transcriptomes based on nucleic acid markerbioRxiv : the preprint server for biology2025.03.14.642805https://www.ebi.ac.uk/biostudies/studies/S-EPMC11957016biostudies-literatureHigh-throughput single cell -omics using semi-permeable capsules.PMC11957016R33 CA278392Zvirblyte JMazutis LJuzenas SGoda KLeonaviciene GSablauskas KBaronas DNorvaisis SMikulenaite VKaseta VGriskevicius LfalseHigh-throughput single cell -omics using semi-permeable capsules.Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput analytical methods and tools that efficiently probe the cellular phenotype and genotype. While recent advancements have enabled various single-cell -omics assays, their broader applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. Extending on our previous work (1) here we present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of high-throughput nucleic acid assays, including digital PCR, genome sequencing, single-cell RNA-sequencing (scRNA-Seq) and FACS-based isolation of individual transcriptomes based on nucleic acid marker2025-01-01T00:00:00Z2025 Mar2026-06-03T03:11:47.431Z2025-07-09T03:05:00.593ZS-EPMC119570164016617410.1101/2025.03.14.642805