biostudies-literatureMorishima KKyoto UniversityJapan Agency for Medical Research and DevelopmentJapan Society for the Promotion of Science595-602https://www.ebi.ac.uk/biostudies/studies/S-EPMC11957415biostudies-literatureUnknown58(Pt 2)Biomacromolecular solutions inevitably contain impurities in addition to the target biomacromolecules. This has been a major obstacle to achieving high-precision solution scattering measurements. To overcome this problem, small-angle X-ray scattering (SAXS) coupled with size-exclusion chromatography (SEC-SAXS) has been developed. This method involves injecting the solution eluted by SEC directly into a measurement cell and conducting SAXS measurements during the elution of the target biomacromolecule. This technique has resulted in a paradigm shift in biomacromolecule solution scattering. Currently, the application of the SEC-SAXS system to small-angle neutron scattering (SANS) is being advanced. However, since the target biomacro-mol-ecules in the sample solution are not only purified butJournal of applied crystallographySize-exclusion chromatography-small-angle neutron scattering system optimized for an instrument with medium neutron flux.PMC11957415JP 19KK0071JP20K06579JP21K18602JP22ama121001j000121K15051Shimizu MInoue RMayumi KOda TSakamoto RNakagawa TMorishima KSugiyama MfalseSize-exclusion chromatography-small-angle neutron scattering system optimized for an instrument with medium neutron flux.Biomacromolecular solutions inevitably contain impurities in addition to the target biomacromolecules. This has been a major obstacle to achieving high-precision solution scattering measurements. To overcome this problem, small-angle X-ray scattering (SAXS) coupled with size-exclusion chromatography (SEC-SAXS) has been developed. This method involves injecting the solution eluted by SEC directly into a measurement cell and conducting SAXS measurements during the elution of the target biomacromolecule. This technique has resulted in a paradigm shift in biomacromolecule solution scattering. Currently, the application of the SEC-SAXS system to small-angle neutron scattering (SANS) is being advanced. However, since the target biomacro-mol-ecules in the sample solution are not only purified but2025-01-01T00:00:00Z2025 Apr2026-06-01T19:08:56.056Z2026-05-19T03:07:27.303ZS-EPMC119574154017096510.1107/S1600576725000779