biostudies-literatureWu PNIDA NIH HHSNHGRI NIH HHSNIGMS NIH HHS527https://www.ebi.ac.uk/biostudies/studies/S-EPMC11958746biostudies-literatureUnknown8(1)Epigenomic mechanisms are critically involved in mediation of genetic and environmental factors that underlie cancer development. Histone modifications represent highly informative epigenomic marks that reveal activation and repression of gene activities and dysregulation of transcriptional control due to tumorigenesis. Here, we present a comprehensive epigenomic and transcriptomic mapping of 18 stage I and II tumor and 20 non-neoplastic tissues from non-small cell lung adenocarcinoma patients. Our profiling covers 5 histone marks including activating (H3K4me3, H3K4me1, and H3K27ac) and repressive (H3K27me3 and H3K9me3) marks and the transcriptome using only 20 mg of tissue per sample, enabled by low-input omic technologies. Using advanced integrative bioinformatic analysis, we uncover canCommunications biologyComprehensive multimodal and multiomic profiling reveals epigenetic and transcriptional reprogramming in lung tumors.PMC11958746R01 GM143940R01 HG009626R01 GM141096R01 DA056187Liu ZWu PZheng LLu CDu YWang WZhou ZfalseComprehensive multimodal and multiomic profiling reveals epigenetic and transcriptional reprogramming in lung tumors.Epigenomic mechanisms are critically involved in mediation of genetic and environmental factors that underlie cancer development. Histone modifications represent highly informative epigenomic marks that reveal activation and repression of gene activities and dysregulation of transcriptional control due to tumorigenesis. Here, we present a comprehensive epigenomic and transcriptomic mapping of 18 stage I and II tumor and 20 non-neoplastic tissues from non-small cell lung adenocarcinoma patients. Our profiling covers 5 histone marks including activating (H3K4me3, H3K4me1, and H3K27ac) and repressive (H3K27me3 and H3K9me3) marks and the transcriptome using only 20 mg of tissue per sample, enabled by low-input omic technologies. Using advanced integrative bioinformatic analysis, we uncover can2025-01-01T00:00:00Z2025 Mar2026-07-15T11:12:51.896Z2025-07-12T03:04:22.126ZS-EPMC119587464016479910.1038/s42003-025-07954-8